BACKGROUND Tissue plasminogen activator (tPA) regulates fibrinolysis and is routinely used as ischemic stroke pharmacotherapy. We hypothesized that brain microvascular tPA expression and barrier properties of endothelial cells are substantially related. METHODS Human brain microvascular endothelial cells were incubated with two agents known to modify cAMP pathways: forskolin and rolipram. We ...

Abstract Background Transcatheter edge-to-edge repair (TEER) with the MitraClip system is an alternative procedure for treatment of severe mitral regurgitation (MR) in high-risk patients who are not suitable conventional surgery and usually performed under general anaesthesia (GA). GA may be associated potential haemodynamic complications. A new approach deep sedation (DS) spontaneous breathing...

TEER is a favorable alternative to surgery in patients with heart failure (HF) and mitral regurgitation (MR), but its effect acute decompensated (ADHF) not well studied. We aim study the short-term outcomes of these using largest US inpatient database. queried 2016-2019 NIS database ICD-10 codes identify all adult MR ADHF. Outcomes who underwent during index hospitalization were compared those ...

Current commercially available in vitro skin models do not fully reproduce the structure and function of native human skin, mainly due to their use animal-derived collagen lack a dynamic flow system. In this study, full-thickness skin-on-a-chip (SoC) system that reproduces key aspects vivo cellular microenvironment is presented. This approach combines production fibroblast-derived matrix (FDM) ...

Homeostatic control of volume within the alveolar spaces of the mammary gland has been proposed to involve a feedback system mediated by serotonin signaling. In this article, we describe some of the mechanisms underlying this feedback based on studies of a human normal mammary epithelial cell line (MCF10A) and mouse mammary epithelium. Mammary serotonin was elevated during lactation and after i...

Treatment of Caco-2-BBe intestinal epithelial cells (BBe) with TNF-α and lymphotoxin-β (LT-β) receptor agonists induced the expression of the TNF receptor superfamily gene TNFRSF9/CD137. In the gut, these cytokines are known to be involved in both inflammatory responses and development of organized lymphoid tissues; thus, it was notable that in CD137-deficient mice Peyer's patch M cells lacked ...

The extensive use of mobile phone communication has raised public concerns about adverse health effects of radiofrequency (RF) electromagnetic fields (EMFs) in recent years. A central issue in this discussion is the question whether EMFs enhance the permeability of the blood-brain barrier (BBB). Here we report an investigation on the influence of a generic UMTS (Universal Mobile Telecommunicati...

Cytokine mediated changes in paracellular permeability contribute to a multitude of pathological conditions including chronic rhinosinusitis (CRS). The purpose of this study was to investigate the effect of interferons and of Th1, Th2, and Th17 cytokines on respiratory epithelium barrier function. Cytokines and interferons were applied to the basolateral side of air-liquid interface (ALI) cultu...

OBJECTIVE Dysfunction of the intestinal epithelial tight junction (TJ) barrier is known to have an important etiologic role in the pathophysiology of heat stroke. N-3 polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), play a role in maintaining and protecting the TJ structure and function. This study is aimed at investigating whether n-3 P...