نتایج جستجو برای: taqman probe

تعداد نتایج: 97576  

Journal: :Journal of clinical microbiology 2004
E M Wagner B L Schmidt A R Bergmann A M Derler E Aberer

We recently described successful molecular diagnosis of B. burgdorferi DNA by nested PCR in urine in patient-derived samples only after extraction with DNAzol (1). The aim of this study was to examine whether Borrelia burgdorferi DNA can also be detected in urine by quantitative one-step real-time PCR (Q-PCR). Q-PCR was performed after extraction with DNAzol (Molecular Research Center Inc., Cin...

Journal: :Veterinary parasitology 2010
Raksha Bhoora Melvyn Quan Linda Franssen Catherine M Butler Johannes H van der Kolk Alan J Guthrie Erich Zweygarth Frans Jongejan Nicola E Collins

A quantitative real-time polymerase chain reaction (qPCR) assay using a TaqMan minor groove binder (MGB) probe was developed for the detection of Babesia caballi infection in equids from South Africa. Nine previously published sequences of the V4 hypervariable region of the B. caballi 18S rRNA gene were used to design primers and probes to target unique, conserved regions. The B. caballi TaqMan...

Journal: :Journal of microbiological methods 2005
Subbarao V Ravva Larry H Stanker

Sensitive real-time sequence detection methods based on two different chemistries were developed for Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of Johne's disease in cattle. One is based on the detection of SYBR Green bound to PCR products and the second method is more specific, detecting the cleavage of a fluorogenic (TaqMan) probe bound to a target sequence during ...

Journal: :Journal of clinical microbiology 2011
Kiyoaki Ito Katsuya Higami Naohiko Masaki Masaya Sugiyama Motokazu Mukaide Hiroaki Saito Yoshihiko Aoki Yo Sato Masatoshi Imamura Kazumoto Murata Hideyuki Nomura Shuhei Hige Hiroshi Adachi Keisuke Hino Hiroshi Yatsuhashi Etsuro Orito Satomi Kani Yasuhito Tanaka Masashi Mizokami

We focused on determining the most accurate and convenient genotyping methods and most appropriate single nucleotide polymorphism (SNP) among four such polymorphisms associated with interleukin-28B (IL-28B) in order to design tailor-made therapy for patients with chronic hepatitis C virus (HCV) patients. First, five different methods (direct sequencing, high-resolution melting analysis [HRM], h...

Journal: :Japanese journal of infectious diseases 2005
Mehmet Yapar Hakan Aydogan Alaaddin Pahsa Bulent A Besirbellioglu Hurrem Bodur Ahmet C Basustaoglu Cakir Guney Ismail Y Avci Kenan Sener Mohammad H Abu Setteh Ayhan Kubar

In this article, the development of a new TaqMan-based one-step real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection and quantification of Crimean-Congo hemorrhagic fever virus (CCHFV) RNA is described. Selected oligos targeting the highly conserved S region of CCHFV were designed by using our oligo design and analysis software, Oligoware 1.0. None of the prime...

Journal: :Clinical chemistry 1998
D Whitcombe J Brownie H L Gillard D McKechnie J Theaker C R Newton S Little

We have developed a method whereby a single TaqMan probe can be used for many PCR reactions. We demonstrate its application as an integrated system for the direct measurement of allele-specific amplicon generation coupled to the suppression of primer-dimer accumulation in PCR. The system uses a 5'-exonuclease assay of amplicon annealed fluorogenic probes that operates in conjunction with the Am...

Journal: :iranian journal of microbiology 0
taghi naserpour-farivar cellular and molecular research center, qazvin university of medical sciences, qazvin, iran. reza najafipour cellular and molecular research center, qazvin university of medical sciences, qazvin, iran. pouran johari cellular and molecular research center, qazvin university of medical sciences, qazvin, iran. masoumeh aslanimehr cellular and molecular research center, qazvin university of medical sciences, qazvin, iran. amir peymani cellular and molecular research center, qazvin university of medical sciences, qazvin, iran. hoasan jahani hashemi cellular and molecular research center, qazvin university of medical sciences, qazvin, iran.

background and objectives: we developed and evaluated the utility of a quadruplex taqman real-time pcr assay that allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. materials and methods: the specificity of the assay was tested using reference strains of vancomycin-resistant and susceptible enterococci. in total, 193 clinical isolates were ...

Journal: :Diagnostic microbiology and infectious disease 2010
Elizabeth Wulff-Burchfield Wiley A Schell Allen E Eckhardt Michael G Pollack Zhishan Hua Jeremy L Rouse Vamsee K Pamula Vijay Srinivasan Jonathan L Benton Barbara D Alexander David A Wilfret Monica Kraft Charles B Cairns John R Perfect Thomas G Mitchell

Rapid, accurate diagnosis of community-acquired pneumonia (CAP) due to Mycoplasma pneumoniae is compromised by low sensitivity of culture and serology. Polymerase chain reaction (PCR) has emerged as a sensitive method to detect M. pneumoniae DNA in clinical specimens. However, conventional real-time PCR is not cost-effective for routine or outpatient implementation. Here, we evaluate a novel mi...

Journal: :Journal of virological methods 2005
Kazuya Shirato Hirotsugu Miyoshi Hiroaki Kariwa Ikuo Takashima

A diagnostic method to distinguish between West Nile virus (WNV) and Japanese encephalitis virus (JEV) based on fluorogenic real-time polymerase chain reaction (TaqMan) assays was developed. To detect WNV and JEV with a single probe, a probe was designed to correspond to sequences in the core protein region that are shared by both viruses. The specificity of this assay depended on the primer se...

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