PCR Conditions: The PCR reaction is carried out in a total volume of 50 /*1 containing approximately 250 ng DNA, 2 units Taq DNA polymerase, 50 pmol of each primer, 200 /M dNTP's, 10 mM Tris-HCl pH 8.3, 50 mM KC1, 1.5 mM MgCl2, 0.001% gelatin. The amplification is performed for 30 cycles with an annealing temperature of 60°C. The amplified product is digested with TaqI and the DNA fragments are...

late-onset alzheimer's disease (ad), a genetically heterogeneous neurodegenerative disorder, is the most common form of dementia in people over 65 years old. the role of vitamin d in neuropsychiatric and neurodegenerative disorders such as ad has been supported by epidemiologic investigations and animal models, as well. we examined the association of the vitamin d receptor (vdr) gene polymorphi...

SOURCE AND DESCRIPTION : The probe that contains exon 1 to 8 was generated from pLDLR3. pLDLR3 contains a full-length LDL receptor cDNA (Yamamoto et at. , 1984) POLYMORPHISM : TaqI identifies a bi-allelic polymorphism with a band of 1.7kb and 0.6kb. FREQUENCY : Studies in 55 unrelated normal Japanese. 1.7Vb fragment 0.69 0.6kb fragment 0.31 NOT POLYMORPHISMS : In 50 individuals no polymorphisms...