نتایج جستجو برای: taq polymerase

تعداد نتایج: 128420  

Journal: :Nucleic acids research 1991
K K Wong M McClelland

When dCTP is replaced by methyl5-dCTP in the polymerase chain reaction some templates cannot be efficiently amplified by Taq polymerase or Vent polymerase using standard cycling parameters. However, this phenomenon can be overcome by increasing the temperature of the denaturation steps to 100 degrees C, or by adding dITP to destabilize the m5dC:dG base pairs. Once the block to amplification of ...

Journal: :BioTechniques 1999
A Louwrier

The polymerase chain reaction (PCR) has revolutionized the molecular biology industry, with profound implications for medical, forensic and research sciences. Prerequisites for this technology to work include the presence of deoxynucleotriphosphates (dNTPs), a relevant buffering system, magnesium chloride, primers and template DNA and finally, the DNA polymerase. Taq polymerase, a thermostable ...

Journal: :Journal of clinical microbiology 2003
Alexandra Heininger Marlies Binder Andreas Ellinger Konrad Botzenhart Klaus Unertl Gerd Döring

DNase I pretreatment of 16S rRNA gene PCR reagents was tested. The DNase I requirement for the elimination of false-positive results varied between 0.1 and 70 IU per master mix depending on the applied Taq polymerase. PCR sensitivity was mostly maintained when 0.1 IU of DNase I was used.

Journal: :Nucleic acids research 1991
Y S Zhu S T Isaacs C D Cimino J E Hearst

The carryover of previously amplified sequences (amplicons) into new PCR reactions is a serious problem. Recently Furrer et al. reported a 'pre-PCR' sterilization technique using DNase I or restriction enzymes for contamination control (1). Unfortunately, these methods require the reaction tube to be re-opened to add target DNA and Taq polymerase following the enzymatic treatment (providing an ...

Journal: : 2021

Objectives . This study investigated the substrate properties of modified derivatives triphosphates purine and pyrimidine deoxynucleosides (5-propynyl-2’-deoxyuridine-5’-triphosphate, 5-propynyl2’-deoxycytidine-5’-triphosphate, 5-methyl-2’-deoxycytidine-5’-triphosphate, N6-methyl-2’-deoxyadenosine-5’-triphosphate) during their simultaneous incorporation in enzymatic reactions (polymerase chain ...

Microsatellites or simple sequence repeats (SSRs) are very effective molecular markers in population genetics, genome mapping, taxonomic study and other large-scale studies. Variation in number of tandem repeats within microsatellite refers to simple sequence length polymorphism (SSLP); but there are a few studies that are showed SSRs replication slippage may be occurred during in vitro amplifi...

Journal: :Chemical communications 2010
Ho-Man Lee Daniel Shiu-Hin Chan Fang Yang Ho-Yu Lam Siu-Cheong Yan Chi-Ming Che Dik-Lung Ma Chung-Hang Leung

Fonsecin B has been identified as stabilizing ligand of c-myc G-quadruplex DNA using high-throughput virtual screening of a natural product database, and inhibited Taq polymerase-mediated DNA extension in vitro through stabilization of the G-quadruplex secondary structure.

Journal: :Applied and environmental microbiology 2004
Charlotta Löfström Rickard Knutsson Charlotta Engdahl Axelsson Peter Rådström

A PCR procedure has been developed for routine analysis of viable Salmonella spp. in feed samples. The objective was to develop a simple PCR-compatible enrichment procedure to enable DNA amplification without any sample pretreatment such as DNA extraction or cell lysis. PCR inhibition by 14 different feed samples and natural background flora was circumvented by the use of the DNA polymerase Tth...

2006
Ma Hongbao

Polymerase chain reaction (PCR) is an in vitro technique to synthesize large quantities of a given DNA molecule that separates the DNA into two complementary strands, uses a peltier heat pump to quickly heat and cool the DNA and uses the Taq polymerase for the synthesis of DNA. Taq is a bacterium that lives by volcanic sulfer jets at the bottom of the ocean where the temperature is very high. F...

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