Background: It has been suggested that organophosphates may inhibit gamma-aminobutyric acid (GABA) metabolism in synaptosomal preparations. In the present investigation, we have assessed the interaction between paraoxon and the GABA system at synaptic level. Methods: Synaptosomes were prepared from male Wistar rats (200-250 g). Cerebral cortex was dissected and homogenized, then centrifuged at ...

background: neurotransmitter release is an essential link in cell communication of the nervous system. many investigations have focused on gamma amino butyric acid (gaba)-ergic neurotransmission, because it has been implicated in the pathophysiology of several central nervous system disorders. to bypass complications related to homo- and heterosynaptic modulation and to avoid indirect interpret...

background: it has been suggested that organophosphates may inhibit gamma-aminobutyric acid (gaba) metabolism in synaptosomal preparations. in the present investigation, we have assessed the interaction between paraoxon and the gaba system at synaptic level.   methods: synaptosomes were prepared from male wistar rats (200-250 g). cerebral cortex was dissected and homogenized, then centrifuged a...

The neuron-specific proteins SNAP-25 (synaptosome-associated protein 25 kDa), synaptobrevin and syntaxin, are localized to presynaptic terminals in mammals and have been found to associate with proteins involved in vesicle docking and membrane fusion. We describe here SNAP-25 cDNA clones from the fruit fly Drosophila melanogaster and the ray Torpedo marmorata. In situ hybridization showed that ...

BACKGROUND Synapse regions in the brain are difficult to isolate and study; resealed nerve terminals (synaptosomes) are a widely used in vitro system for the study of neurotransmission, but nonsynaptosomal elements in the homogenate complicate data interpretation. With the goal of quantitative analysis of pathways leading to synapse loss in neurodegenerative disease, we have developed a method ...

Serotonin 5-hydroxytryptamine type 3 receptors (5HT3R) are Ca2+-permeant, non-selective cation channels that have been localized to presynaptic terminals and demonstrated to modulate neurotransmitter release. In the present study the effect of 5-HT on GABA release in the hippocampus was characterized using both electrophysiological and biochemical techniques. 5-HT elicited a burst-like, 6- to 1...