نتایج جستجو برای: sybr green

تعداد نتایج: 140135  

Journal: :The Tohoku journal of experimental medicine 2006
Yasushi Onishi Shin-Ichiro Mori Akiko Higuchi Sung-Won Kim Takahiro Fukuda Yuji Heike Ryuji Tanosaki Toshio Minematsu Yoichi Takaue Takeshi Sasaki Koh Furuta

Cytomegalovirus (CMV) infection is an important cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation. Therefore, preemptive ganciclovir therapy based on early detection of CMV reactivation is widely used to prevent CMV disease. Real-time polymerase chain reaction (PCR) has been widely used for monitoring CMV reactivation as well as the antigenemia assay that...

2015
Galina E. Zemtsova Merrill Montgomery Michael L. Levin

Studies on the natural transmission cycles of zoonotic pathogens and the reservoir competence of vertebrate hosts require methods for reliable diagnosis of infection in wild and laboratory animals. Several PCR-based applications have been developed for detection of infections caused by Spotted Fever group Rickettsia spp. in a variety of animal tissues. These assays are being widely used by rese...

Journal: :Journal of medical microbiology 2014
Filipe Cerca Angela França Begoña Pérez-Cabezas Virgínia Carvalhais Adília Ribeiro Joana Azeredo Gerald Pier Nuno Cerca Manuel Vilanova

Staphylococcus epidermidis is the most commonly isolated aetiological agent of nosocomial infections, mainly due to its ability to establish biofilms on indwelling medical devices. Detachment of bacteria from S. epidermidis biofilms and subsequent growth in the planktonic form is a hallmark of the pathogenesis of these infections leading to dissemination. Here we showed that S. epidermidis cell...

2015
Rafael Felipe da Costa VIEIRA Odilon VIDOTTO Thállitha Samih Wischral Jayme VIEIRA Ana Márcia Sá GUIMARAES Andrea Pires dos SANTOS Naíla Cannes NASCIMENTO Nelson Jesse Rodrigues dos SANTOS Thiago Fernandes MARTINS Marcelo Bahia LABRUNA Mary MARCONDES Alexander Welker BIONDO Joanne Belle MESSICK

The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR gre...

Journal: :Journal of infection in developing countries 2014
Subhra Subhadra Subrat Kumar Veluvarthy V S Suryanarayana Daggupati Sreenivasulu

INTRODUCTION Bluetongue (BT), a vector-borne viral disease, primarily affects sheep. Of the 26 serotypes of BTV identified so far, 22 are reported to be circulating in India. Due to an increase in vector population and delays in disease diagnosis, the BT control program heavily relies on rapid and confirmatory diagnosis. Polymerase chain reaction (PCR)-based real-time detection assays may be an...

2015
Qin Zhao Sha Xie Yani Sun Yiyang Chen Jiming Gao Huiya Li Xinjie Wang Shahid Faraz Syed Baoyuan Liu Lizhen Wang Gaiping Zhang En-Min Zhou

BACKGROUND Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease, as well as hepatitis-splenomegaly syndrome in chickens. To date, conventional reverse transcriptase polymerase chain reaction (RT-PCR) and nested RT-PCR methods have been used for the diagnosis of avian HEV infection in chickens. However, these assays are time consuming, inconvenient, and canno...

Journal: :Journal of microbiology and biotechnology 2015
Min Seok Cho Duck Hwan Park Tae-Young Ahn Dong Suk Park

The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYB...

2014
Ken-Ichi Hanaki Fumio Ike Ayako Kajita Wataru Yasuno Misato Yanagiba Motoki Goto Kouji Sakai Yasushi Ami Shigeru Kyuwa

A one-step SYBR Green I real-time RT-PCR assay was developed for the detection and quantification of a broad range of murine noroviruses (MNVs). The primer design was based on the multiple sequence alignments of 101 sequences of the open reading frame (ORF)1-ORF2 junction of MNV. The broad reactivity and quantitative capacity of the assay were validated using 7 MNV plasmids. The assay was compl...

2009
M. Abou El Hassan R. Bremner

Chromosome conformation capture (3C) is a powerful tool to study DNA looping. The procedure generates chimeric DNA templates after ligation of restriction enzyme fragments juxtaposed in vivo by looping. These unique ligation products (ULPs) are typically quantified by gel-based methods, which are practically inefficient. Taqman probes may be used, but are expensive. Cycle threshold (Ct) determi...

Journal: :Journal of forensic sciences 2007
Jaiprakash G Shewale Elaine Schneida Jonathan Wilson Jerilyn A Walker Mark A Batzer Sudhir K Sinha

The human DNA quantification (H-Quant) system, developed for use in human identification, enables quantitation of human genomic DNA in biological samples. The assay is based on real-time amplification of AluYb8 insertions in hominoid primates. The relatively high copy number of subfamily-specific Alu repeats in the human genome enables quantification of very small amounts of human DNA. The olig...

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