We have investigated the effect of surface roughness on the sensitivity of conventional and nanowire-based surface plasmon resonance (SPR) biosensors. The theoretical research was conducted using rigorous coupled-wave analysis with Gaussian surface profiles of gold films determined by atomic force microscopy. The results suggest that, when surface roughness ranges near 1 nm, the sensitivity of ...

The aim of this research is to characterize the optical properties of heavy metal ions (Hg, Cu, Pb and Zn) using surface plasmon resonance (SPR) technique. Glass cover slips, used as substrates were coated with a 50 nm gold film using sputter coater. The measurement was carried out at room temperature using Kretchmann SPR technique. When the air medium outside the gold film is changed to heavy ...

Deposition of behenic acid (B.A) mono and bilayers onto gold coated surfaces was performed by Langmuir-Blodget dip-casting technique. Surface Plasmon resonance (SPR) and ellipsometry methods were employed for investigation of the monolayer and bilayer films. The adsorption of the biologically important molecule Concanavalin A (Con A) from bulk solution to these monolayers and bilayers as we...

According to circumstance of kerman sarcheshmeh cooper complex that its anode slime is mainly consisted of Cu, Ag, Au, Pb and Se. In this work, recovery of silver from anode slime and subsequent synthesis of silver nanoparticles from leaching solution was made. Silver was separated from anode slime by using of HNO3 and HCl. X-Ray fluorescence spectroscopy (XRF) was used to characterize anode sl...

We describe a non-scan and real-time multichannel angular surface plasmon resonance (SPR) imaging method. We demonstrate experimentally, with multiple line shaped light illuminations to construct multi-imaging channels, that an image captured with an area detector can probe the surface plasmons with different field distributions. Thus, it provides a fixed optical-sensing module measuring the sp...

Light response of a pachira aquatica’s leaf at room temperature was investigated with a strong p-wave electromagnetic field by utilizing a surface plasmon resonance (SPR) based sensor technique of angular interrogation method for biological and chemical sensing. The essential principle of SPR biosensing is based on measurement of the change regarding leaf’s microstructure against the sensor’s m...

A novel surface enzymatic reaction scheme that amplifies the optical response of RNA microarrays to the binding of complementary DNA is developed for the direct detection and analysis of genomic DNA. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from DNA-RNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonan...

A novel hybrid spectroscopic technique is proposed, combining surface plasmon resonance (SPR) with surface-enhanced Raman scattering (SERS) microscopy. A standard Raman microscope is modified to accommodate the excitation of surface plasmon-polaritons (SPPs) on flat metallic surfaces in the Kretschmann configuration, while retaining the capabilities of Raman microscopy. The excitation of SPPs i...

The present study introduces a novel curve-fitting algorithm for surface plasmon resonance (SPR) curves using a self-constructed, wedge-shaped beam type angular interrogation SPR spectroscopy technique. Previous fitting approaches such as asymmetric and polynomial equations are still unsatisfactory for analyzing full SPR curves and their use is limited to determining the resonance angle. In the...

The progressively improved sensing sensitivity (Î?λSPR/Î?n, nm/RIU) to detect the refractive index is observed on SPR platform of an Au-covered epoxy gratings in increase potential cycling a typical three-electrode cell. Here, DVD-R optical disc was used as structure template prepare gratings, and newly formed reverse track pitch substrate working electrode directly aqueous sulfuric acid solut...