BACKGROUND Tumour heterogeneity and resistance to systemic treatment in urothelial carcinoma (UC) may arise from cancer stem cells (CSC). A recent model describes cellular differentiation states within UC based on corresponding expression of surface markers (CD) and cytokeratins (CK) with CD90 and CK14 positive cells representing the least differentiated and most tumourigenic population. Based ...

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We studied the expression of cell surface antigens associated with myeloid and lymphoid leukemias on bone marrowderived blast cells from 339 patients with newly diagnosed de novo acute myeloid leukemia (AML) enrolled on Cancer and Leukemia Group B (CALGB) chemotherapy protocols. Surprisingly, of 211 cases studied for the expression of CD2 (T-cell marker, sheep erythrocyte binding receptor for T...

The fate of two recently described human B lymphocyte-specific antigens (B1 and B2) was studied after B-cell activation in vivo and in vitro. Whereas both B1 and B2 were present on virtually all B cells from normal lymph nodes, B2 was absent from approximately 50% of B cells from hyperplastic lymph nodes. When B cells from spleen, tonsil, or peripheral blood were stimulated in vitro with pokewe...

An understanding of the distinct families of molecules present on different cells of the immune system provides the tools for distinguishing these cell types in both diagnosis and therapy, as well as understanding the molecular basis for many lymphoid cell functions. Diagnostic MARKERS for T-cells include the T-cell receptor (TcR) with its associated CD3 signaling complex as well as CD5 and the...

Migration from blood to tissue modulates eosinophil function, possibly through interactions with endothelial cells. The effects of contact with and migration through endothelial cells on eosinophil expression of surface markers and release of leukotriene C4 were evaluated. A small proportion (2.6%) of eosinophils spontaneously migrated through endothelial cell monolayers. Activation of endothel...

PERIPHERAL BLOOD LYMPHOCYTES FROM NORMAL SUBJECTS AND PATIENTS WITH VIRAL AND BACTERIAL INFECTIOUS DISEASES WERE EXAMINED FOR THE PRESENCE OF THREE SURFACE MARKERS: (i) surface immunoglobulins, (ii) receptor for C3 complement component (EAC test), and (iii) spontaneous binding of sheep red blood cells (E rosette formation). The first two markers are used to detect bone marrow-derived lymphocyte...

By using the two criteria (a) high density of immunoglobulin determinants on the cell surface and (b) presence of receptors for C'3 on the cell surface for defining bone marrow-derived lymphocytes, it is indirectly shown that all or at least a major population of human thymus-derived lymphocytes under certain conditions will form nonimmune rosettes with sheep red blood cells (SRBC). Almost all ...

Leukemic cells from 20 patients with chronic lymphocytic leukemia (CLL) and 60 patients with acute lymphocytic leukemia (ALL) were studied for T- and B-lymphocyte cell surface membrane markers. B-cell markers included surface membrane immunoglobulin, erythrocyte-antibody complement rosette formation, and B-cell (a-like or HLA-DR) antigens detected by a B-cell antiserum. T-cell markers included ...