Soybean plant responses to Heterodera glycines (H. glycines) race 3 infection were compared between resistant (Peking, LiaoK89102 and Franklin) and susceptible cultivars (Liao dou 10 and Kaiyu 10) at 10, 17, 20, and 23 days after inoculation (DAI). After inoculation with H. glycines race 3, the isozymes of peroxidase, polyphenol oxidase, superoxide dismutase, and catalase from the soybean roots...

Background: Blood is permanently exposed to oxidation stress and therefore has a high antioxidants capacity. Many different factors increasing the demand for the antioxidant capacity can be observed in the stored blood of donors. Consequently, damage to erythrocytes by free radicals may occur. So it is useful to control the alternation of anti-oxidant enzymes in stored blood at different days o...

Superoxide dismutase catalyzes the decay of 0~~, generated by pulse radiolysis. The enzymatic reaction was 6rst order with respect to enzyme and to 0~~ and the rate constant, which was 2.3 X lo9 M-' s-l at pH 7.0 and ZO-25”, was not much affected by pH, in the range 4.8 + 9.5. Boiling the enzyme or reversibly removing its copper prosthetic group caused loss of catalytic activity. EDTA had no ef...

The activities of superoxide dismutase and glutathione peroxidase in the red cells of normal and acatalasemia bloods were compared. Superoxide dismutase activity in the red cells of Japanese acatalasemia blood was higher than in normal control, while glutathione peroxidase activity remained within normal limits. Cyanide sensitivity of superoxide dismutase in the red cells of acatalasemia blood ...

The nonenzymatic glycosylation (glycation) of Cu-Zn-superoxide dismutase led to gradual inactivation of the enzyme (Arai, K. Iizuka, S., Tada, Y., Oikawa, K., and Taniguchi, N. (1987) Biochim. Biophys. Acta 924, 292-296). The purified superoxide dismutase from human erythrocytes comprises both glycated and nonglycated forms. The nonglycated Cu-Zn-superoxide dismutase was isolated by boronate af...

Isoniazid (INH) activation in vitro is associated with reduction of the mycobacterial ferric KatG catalase-peroxidase by hydrazine and reaction with O2 to form an oxyferrous enzyme complex. Since this complex could also form directly via reaction of ferric KatG with superoxide, intracellular activation might be responsive to superoxide concentration. When Mycobacterium smegmatis carrying the M....

The effects of heat and NaCl on the activity of superoxide dismutase from Staphylococcus aureus were examined. A linear decrease in superoxide dismutase activity occurred when S. aureus MF-31 cells were thermally stressed for 90 min at 52% C in 100 mM potassium phosphate buffer (pH 7.2). After 20 min of heating, only 5% of the superoxide dismutase activity was lost. Heating for 60, 90 and 120 m...

the human erythrocyte is a rich raw material for the purification of cu-zn superoxide dismutase (sod). we applied a simple and rapid procedure for the purification of sod from human erythrocytes by ion exchange chromatography. the purified sod had a specific activity of 2285.6 u/mg protein and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sd...