The interplay between nitric oxide and superoxide to form peroxynitrite is a major biological process that competes in a remarkably subtle fashion with superoxide dismutase in vivo. The complex interactions between superoxide, nitric oxide reveals some extraordinary features about the difficulties of effectively scavenging of superoxide in a biological system. The discovery of dominant mutation...

Cytochrome c oxidase (CCO) is the Cu-dependent, terminal respiratory complex of the mitochondrial electron transport chain. Inhibition of CCO can promote oxidative stress by increasing mitochondrial production of reactive oxygen species (ROS). Because mitochondria have an important role in apoptosis as both a target and source for ROS, enhanced ROS production resulting from inhibition of CCO by...

Indian Ber contain good source of ascorbic acid and total phenolics. Despite being rich in antioxidants it is an underutilized fruit. Effect of processing on antioxidant activity of ber fruit was studied. Antioxidant activity of ber fruit was evaluated by different methods viz. DPPH radical activity, reducing power assay, superoxide anion radical activity, TBARS, total phenolic content and tota...

Aim: This study was aimed to investigate the levels of antioxidant markers (paraoxonase, superoxide dismutase, glutathione peroxidase, and thiol) oxidative stress (advanced oxidation protein products, xanthin oxidase, nitric oxide, malondialdehyde, 8-hydroxydeoxyguanosine) in pregnant women with chronic hepatitis B C. Material Method: Sixty last trimester, 20 whom had B, another C, remaining we...

1. We describe a rapid and reliable technique for the assessment of basal nitric oxide release in clinical situations, using peripheral blood polymorphonuclear leucocytes isolated by a single-step density gradient procedure. The assay is based on the quantitative conversion of oxyhaemoglobin to methaemoglobin by nitric oxide. We have further examined the ability of these cells to respond to var...

conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...

Introduction: Iron plays an important role in physiological processes as a trace element. Today, iron oxide nanoparticles have attracted extensive attention due to their super paramagnetic properties and a variety of potential applications in many fields. The main objective of this study was to evaluate in vitro and in vivo toxic effects of the iron oxide nanoparticles on L929 cell line, kidney...

We developed Cu-deficient, -sufficient and -super nutrition mice models by feeding them with diet containing 1.68, 11.72 or 51.69 mg of Cu/kg for 28 days, respectively. Then, the mice were treated to (-)-epigallocatechin-3-gallate (EGCG, 750 mg/kg BW) by oral in order to assess the acute toxicity of the drug. Following EGCG treatment, the survival rates were 12.5%, 50% and 100% in the Cu-defici...