Maltose-maleimide was synthesized as a potential affinity label for the facilitative hexose carrier with selectivity for exofacial sulphydryl groups. This reagent, although probably a mixture of isomers, did not significantly penetrate the plasma membrane of human erythrocytes at concentrations below 5 mM at 37 degrees C. When allowed to react to completion, it irreversibly inhibited the uptake...

In this paper, a dry heat method is used to modify wheat gluten, and the effects of dry heat on the enzymatic hydrolysis and structure of wheat gluten are investigated. The results show that the degree of hydrolysis of wheat gluten after dry heating increased from 20 to 30 % within 1 h of hydrolysis. Meanwhile, the percentage of the fraction with Mr<1000 Da in the obtained hydrolysate increased...

The aim of the present study was to determine changes in oxidative status plasma sows during periparturient period using markers lipid and protein peroxidation intensity. Plasma from 12 pregnant gilts collected on days 14 1 before parturition 1, 7 postpartum used study. As indicators alterations status, concentrations thiobarbituric acid reactive substances (TBARS) sulphydryl groups (SH groups)...

The protein component of a number of enzyme systems contains SH groups which are either freely reacting or sluggish. The freely reacting SH groups give the nitroprusside test in the native protein, are easily oxidized by mild oxidizing agents and alkylating agents in low concentrations (iodoacetate,mustardgas, diphosgene, cyanogenchloride), and readily form mercaptides. On denaturation, the slu...

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a typical X-linked enzymopathy causing severe haemolytic anaemia in males, and mild to moderate anaemia in homozygous females. Haemolysis due to G6PD deficiency in patients with type 1 diabetes mellitus (T1DM) has been principally reported in males, but is uncommon. During the last 10 years 2 girls with an unknown incomplete G-6-PD deficien...

Oxidative stress is one of the major causes of male infertility; it damages spermatogenic cells, the spermatogenic process and sperm function. Recent advances in redox biology have revealed the signalling role of reactive oxygen species (ROS) that are generated by cells. While highly reactive oxidants, such as the hydroxyl radical, exert largely deleterious effects, hydrogen peroxide can feasib...

The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent conditions, together with gel electrophoresis studies of DNH modified with DTNB, sh...

Proton transfer reactivity of isolated charge states of the protein hen egg-white lysozyme shows that multiple distinct conformations of this protein are stable in the gas phase. The reactivities of the 9+ and 10+ charge state ions, formed by electrospray ionization of "native" (disulfide-intact) and "denatured" (disulfide-reduced) solutions, are consistent with values calculated for ions in th...