Trinitrobenzenesulfonic acid (TNBS) may be used for quantifying dilute solutions of protein. The trinitrophenyl protein-sulfite corn plexes resulting from reaction of TNBS with protein in the presence of sulfite are highly colored and exhibit maximum spectral absorbance at 420 nm. The TNBS protein-sulfite complex is formed by a simple two.stage condensation and complexation reaction. Protein in...

Sulfite and sulfide share several similarities in terms of chemical properties, such as nucleophilic and reducing reactivities. Therefore, they may disturb the detection of each other. In order to discriminate between these two kinds of sulfur-containing species, a new probe -N3 was developed, in which para-azidobenzenyl ketone was covalently incorporated to a coumarin fluorophore linked by a C...

This study investigated sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) pretreatment and subsequent enzymatic digestibility of undigested dairy manure to preliminarily assess its potential use as an inexpensive feedstock for cellulosic bioethanol production. The sulfite pretreatment was carried out in a factorial analysis using 163 to 197 °C for 3 to 37 min with 0.8% t...

The aerobic oxidation of sulfite thus provides a magnified manometric response to the chain initiating event. Advantage was taken of this circumstance to develop an ultrasensitive assay for milk xanthine oxidase (5). The data obtained were consistent with the hypothesis that xanthine oxidase, when acting on purine substrates, initiates the aerobic oxidation of sulfite by the generation of free ...

The enhancement of CO2 fixation in isolated, intact spinach chloroplasts by ascorbate or by low sulfite concentrations (less than 1 mM) is strongly reduced or even abolished by the addition of superoxide dismutase (SOD). By the use of 35SO3(2-) it is demonstrated that the rate of sulfate formation is much lower than the sulfite induced increase in CO2 fixation. This indicates that the superoxid...

BACKGROUND Wine yeasts can produce undesirable sulfur compounds during alcoholic fermentation, such as SO2 and H2S, in variable amounts depending mostly on the yeast strain but also on the conditions. However, although sulfur metabolism has been widely studied, some of the genetic determinants of differences in sulfite and/or sulfide production between wine yeast strains remain to be identified...

BACKGROUND Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for process design and optimization. Softwood biomass is an abundant resource in many parts of the world, including Scandinavia, but efficient pretreatment and subsequent enzymatic hydr...

The aerobic oxidation of sulfite thus provides a magnified manometric response to the chain initiating event. Advantage was taken of this circumstance to develop an ultrasensitive assay for milk xanthine oxidase (5). The data obtained were consistent with the hypothesis that xanthine oxidase, when acting on purine substrates, initiates the aerobic oxidation of sulfite by the generation of free ...

The molybdenum-containing enzyme sulfite oxidase catalyzes the conversion of sulfite to sulfate, the terminal step in the oxidative degradation of cysteine and methionine. Deficiency of this enzyme in humans usually leads to major neurological abnormalities and early death. The crystal structure of chicken liver sulfite oxidase at 1.9 A resolution reveals that each monomer of the dimeric enzyme...