In this study, the effect of different isolation techniques on the isolated proteins from pigeon pea was investigated. Water, methanol, ammonium sulfate, and acetone were used for the precipitation of proteins from pigeon pea. Proximate composition, and antinutritional and functional properties of the pigeon pea flour and the isolated proteins were measured. Data generated were statistically an...

A novel method to characterize the organic composition of aerosol particles has been developed. The method is based on organic vapor interaction with aerosol particles and it has been named an Organic Tandem Differential Mobility Analyzer (OTDMA). The OTDMA method has been tested for inorganic (sodium chloride and ammonium sulfate) and organic (citric acid and adipic acid) particles. Growth cur...

dissolved organic nitrogen (don) is an important constituent of dissolved organic matter (dom). it has a considerable effect on total dissolved soil nitrogen and it is very important as a nitrogen source of many aquatic and terrestrial ecosystems. dissolved inorganic nitrogen, which is another form of total soil dissolved nitrogen, includes no3, no2 and nh4, which is very important for plant nu...

A protein purification procedure is required to obtain high-value recombinant injectable vaccine proteins produced in plants as a bioreactor. However, existing purification procedures for plant-derived recombinant proteins are often not optimized and are inefficient, with low recovery rates. In our previous study, we used 25-30% ammonium sulfate to precipitate total soluble proteins (TSPs) in p...

At 0”, native tetrameric rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (7.4 S) undergoes a reversible inactivation and dissociation into 4.4 S dimers or 3.2 S monomers, depending upon the species of salt present and upon the protein concentration. These dissociation processes have been studied using sucrose density gradient centrifugation. With 0.10 mg per ml of enzyme in 0.15 M ammoni...