The effluents of wastewater treatment plants (WWTPs) are major contributors nutrients, microbes—including those carrying antibiotic resistance genes (ARGs)—and pathogens to receiving waterbodies. effect the effluent a small-scale activated sludge WWTP treating municipal on composition and abundance microbial community as well resistome in sediment water stream river was studied using metagenome...

Determining the prevalence of antimicrobial resistance (AMR) in non-clinical settings is vital for better management global AMR crisis. Untreated and even treated wastewaters are important sources that release into environment. Methodologically, it difficult to generate a comprehensive situ profile antibiotic gene hosts. Here, we used epicPCR (emulsion, paired isolation, concatenation PCR) as c...

AVALIAÇÃO DE ELEMENTOS GENÉTICOS RESISTÊNCIA A ANTIBIÓTICOS EM ÁREA SOB INFLUÊNCIA DA FERTIRRIGAÇÃO COM EFLUENTE TRATADO UMA GRANJA SUINOCULTURA GERUSA LEITE CAETANO1; ANDRÊSSA REZENDE PEREIRA2 E SILVANA QUEIROZ SILVA3 1 Programa de Pós-Graduação em Engenharia Ambiental, Universidade Federal Ouro Preto, Campus Universitário Morro do Cruzeiro, 35400-000, Minas Gerais, Brasil, gerusa.caetano@alun...

Antibiotic resistance genes (ARGs) have been reported to threaten the public health of beachgoers worldwide. Although ARG monitoring and beach guidelines are necessary, substantial efforts required for sampling analysis. Accordingly, in this study, we predicted ARGs occurrence that primarily found on coast after rainfall using a conventional long short-term memory (LSTM), LSTM-convolutional neu...

Salmonellosis is caused by a thousand serotypes of Salmonella enterica. The sour taste inherent to Nham leads people believe that this fermented ground pork dish safe from pathogenic microorganisms. aim study was evaluate the prevalence, serotype, drug susceptibility, and antimicrobial resistance (AMR) genes spp. in homemade recipes Nham. There were 52 samples different makers 3 northeastern pr...

ABSTRACT Background and Objectives: One major source of antibiotic contamination in the sea is from aquaculture. We monitored concentration commonly used classes resistance genes ( tet (M), sul1, sul2 sul3 ) aquaculture farms Peninsular Malaysia. Methods: Antibiotic residues were quantified using high-performance liquid chromatography real-time PCR respectively. Risk quotients European technica...

OBJECTIVES To characterize a multidrug-resistant Aeromonas hydrophila isolate (CVM861) that possesses a high-level macrolide inactivation gene cluster (mphA-mrx-mphR), previously only reported in Escherichia coli. METHODS PCR fragment length mapping, gene sequencing and Southern blotting were used to map the mphA-mrx-mphR gene cluster and flanking elements in CVM861. Conjugation experiments w...

The term enzibiotic was coined from two words, enzyme and antibiotic and usually refers as the bacteriophage enzymes that attack the cell wall of bacteria with lyses [1]. However, enzybiotic present in bacteria, bacteria infected phages and in body fluids like tears, saliva and animal mucous [2]. Antibiotics had used 80 years with success to eradicate pathogenic bacteria like Escherichia, Klebs...

Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. Presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximate...

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens...