We attempted to determine the number of sperm cells required for genotyping of one microsatellite marker. The crude genomic DNA extracted from about 760 or more sperm cells gave sufficient quantity of PCR product using a 20 microl-scale PCR. We also studied the effects of non-ionic detergents on extraction of crude sperm genomic DNA. PCR products amplified with the crude sperm genomic DNA extra...

The idea of dimension-reduction without loss of information can be quite helpful for guiding the construction of summary plots in regression without requiring a pre-specified model. Focusing on the central subspace, we investigate such “sufficient” dimension-reduction in regressions with a binary response. Three existing methods, SIR and pHd and SAVE, and one new method DOC are studied for thei...

Chen et al. (2010) propose a unified method – coordinate-independent sparse estimation (CISE) – that is able to simultaneously achieve sparse sufficient dimension reduction and screen out irrelevant and redundant variables efficiently. However, its attractive features depend on appropriate choice of the tuning parameter. In this note, we re-examine the Bayesian information criterion (BIC) in su...

Luikov’s theory of heat and mass transfer provides a framework to model drying porous materials. Coupled partial differential equations governing themoisture and heat transfer can be solved using numerical techniques, and in this paper we solve them analytically in a setting suitable for industrial drying situations. We discuss the nature of the solutions using the physical properties of Pinus ...