Terminal saccharide sequences in rat photoreceptor cell surface glycoconjugates were characterized. Lectin cytochemistry and electron microscopy were used for preembedding cytochemical localization of surface carbohydrates. Neuraminidase digestion was employed for the exposure of penultimate saccharides in sialoglycoconjugates. Isolated rat retinas were incubated with ferritin-labeled wheat ger...

Terminal saccharide sequences in rat photoreceptor cell surface glycoconjugates were characterized. Lectin cytochemistry and electron microscopy were used for preembedding cytochemical localization of surface carbohydrates. Neuraminidase digestion was employed for the exposure of penultimate saccharides in sialoglyconjugates. Isolated rat retinas were incubated with ferritin-labeled wheat germ ...

Three lectins, wheat germ agglutinin (WGA), soybean agglutinin (SBA) and Ricinis communis agglutinin I (RCA), were used to study the basement membrane of developing chick lungs. Thinning of the basement membrane at the tips of newly formed bronchi was visualized with all three lectins, but was particularly evident using SBA. Control sections established the ability of the lectins to stain hyalu...

We have recently described "fracture-label" techniques that permit direct cytochemical labeling of freeze-fractured cells. We report here the use of fracture-labeling to investigate the distribution and partition of wheat germ agglutinin (WGA) receptor sites over the protoplasmic and exoplasmic plasma membrane faces of freeze-fractured human thymus-derived (T) lymphocytes. All exoplasmic faces ...

Following the initial observation about the exsistence of plant lectins-immunoreactive material in different rat and human tissues (D ebb age et al. 1993), we isolated a specific wheat germ agglutinin (WGA)-immunoreactive (IR) protein from human placenta by combination of ion-exchange and immunoaffinity chromatography. It was characterized as 66 kD glycoprotein exhibiting calcium-dependent, car...

The properties of highly purified bovine cardiac sarcolemma subfractionated with the lectin, wheat-germ agglutinin (WGA) were studied. Two different membrane subfractions were isolated, one which was agglutinated in the presence of 1.0 mg of WGA/mg of protein (WGA+ vesicles) and a second fraction which failed to agglutinate (WGA- vesicles). These two membrane fractions had quantitatively differ...

T cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. When assayed with 125I- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (WGA), a weakly binding and a strongly binding subpopulation could be distinguished. These T-cell subpopulations were fractionated on columns charged with WGA, con...

A series of tetravalent glycocyclopeptides functionalized with GlcNAc was synthesized using copper(i)-catalysed alkyne-azide cycloaddition, oxime ligation and thiol-ene coupling. The binding ability of these compounds towards wheat germ agglutinin was studied by a competitive ELLA test and ITC experiments. While all compounds were able to inhibit WGA binding to GlcNAc-polymer coated surfaces at...

The characteristics of the surface of guinea pig megakaryocytes were investigated with wheat germ agglutinin (WGA). Purified guinea pig megakaryocytes and platelets were incubated with WGA conjugated with rhodamine, cytocentrifuged, and then exposed to Chromomycin A3 for the assessment of ploidy. The fluorescence emission of the DNA-Chromomycin complex was similar to that of fluorescein, and th...