AIM This study was planned to study the genotypes of verocytotoxigenic Escherichia coli (VTEC) in fecal samples of deer due to its public health significance. MATERIALS AND METHODS A total of 160 fecal samples of deer were taken from Mathura district and Kanpur Zoo and screened for VTEC genes by polymerase chain reaction (PCR). RESULTS All fecal samples were positive for E. coli. All the E....

today, it is nearly 25 years past from investigation of shiga toxigenic escherichia coli (stec) which is able to produce shiga toxins and cause different gastroenteritis. since incidence of gastroenteritis due to stec is increasing, it's necessary to develop rapid, specific and accurate procedures like pcr. in this study, we used pcr method to detect and identify stec in cultures of 55 escheric...

pathogens can be transmitted to the humans through the consumption of contaminated local dairy products such as cheese and, thus, cause pathogenic diseases. shiga toxin produced by escherichia coli can cause mild watery diarrhea as well as serious complications such as hemorrhagic colitis, and hemolytic uremic syndrome and may even lead to death. the present study was conducted to investigate t...

shiga toxin-producing escherichia coli (stec) are food-borne pathogens primarily associated with the consumption of contaminated ground beef and are an important food safety concern worldwide. stec has been found to produce a family of related cytotoxins known as shiga toxins (stxs). shiga toxins have been classified into two major classes, stx1 and stx2. a single strains of stec can produce st...

Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx1, and stx2.

This study presents three genomes of O91:H21 isolates, two from hemolytic uremic syndrome patients and one of porcine origin. Genome analyses reveal that one of the human isolates contains both Shiga toxin-encoding genes (stx1 and stx2), and all three isolates contain putative adhesin (iha and eaeH) and antibiotic resistance (ampC) genes.