Previously, it was shown that β-ketoacyl-coenzyme A synthase ECERIFERUM6 (CER6) is necessary for the biosynthesis of very-long-chain fatty acids with chain lengths beyond C₂₈ in tomato (Solanum lycopersicum) fruits and C₂₆ in Arabidopsis (Arabidopsis thaliana) leaves and the pollen coat. CER6 loss of function in Arabidopsis resulted in conditional male sterility, since pollen coat lipids are re...

The cellular fatty acid composition of Mycobacterium vaccae JOB5 and Mycobacterium convolutum R22 was examined after growth on n-alkanes and compared with the fatty acids of the organisms after growth on 1-chlorohexadecane and 1-chlorooctadecane. Growth on n-alkanes resulted in normal fatty acid profiles. Mass spectral analyses indicated that, after growth on the terminally chlorinated n-alkane...

Seven crude oils of known source and maturity, representing a natural sequence of increasing degree of biodegradation, were collected from reservoirs in the Liaohe Basin, NE China, in an effort to determine the magnitude and direction of isotopic shift of carbon and hydrogen in individual n-alkanes during microbial degradation. The results show that biodegradation has little effect on the carbo...

This study aimed to assess the use of very long-chain fatty acids (VLCFA) as an alternative or a complement to n-alkane markers for estimation of diet composition of goats fed 7 different diets, composed of different proportions of herbaceous (Lolium perenne and Trifolium repens) and heathland woody species (Erica umbellata, Erica cinerea, Calluna vulgaris, Erica arborea, and Ulex gallii), in a...

This work puts forward a toolkit that enables the conversion of alkanes by Escherichia coli and presents a proof of principle of its applicability. The toolkit consists of multiple standard interchangeable parts (BioBricks)(9) addressing the conversion of alkanes, regulation of gene expression and survival in toxic hydrocarbon-rich environments. A three-step pathway for alkane degradation was i...

Microbial treatment of swine fecal waste to generate long-chain linear alkanes after fast pyrolysis" (2010).

Abstract. Despite the importance of soil organic matter (SOM) in global carbon cycle, there remain many open questions regarding its formation and preservation. The study individual compound classes that make up SOM, such as lipid biomarkers including n-alkanes, can provide insight into cycling bulk SOM. While studies biomarkers, particularly have increased number past few decades, only a limit...

Selective oxyfunctionalization of non-activated C–H bonds remains a major challenge in synthetic chemistry. The biocatalytic hydroxylation by cytochrome P450 monooxygenases (CYPs), however, offers catalysis with high regio- and stereoselectivity using molecular oxygen. CYP153s are class CYPs known for their selective terminal n-alkanes microorganisms, such as the bacterium Alcanivorax dieselole...