This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by ...

BACKGROUND Viral hemorrhagic fevers (VHFs) are a group of animal and human illnesses that are mostly caused by several distinct families of viruses including bunyaviruses, flaviviruses, filoviruses and arenaviruses. Although specific signs and symptoms vary by the type of VHF, initial signs and symptoms are very similar. Therefore rapid immunologic and molecular tools for differential diagnosis...

a water snake (natrix natrix) was referred to the small animal teaching hospital, faculty of veterinary medicine, university of tehran. it had two subcutaneous masses. radiographic and ultrasonographic images revealed the presence of two subcutaneous abscesses. the abscesses were removed surgically and specimens were examined by conventional microbial culture and multiplex pcr. inv-a universal ...

rotaviruses group a are the major cause of diarrhea in calves under one month and every year causes enormous economic losses. serological and molecular techniques can be used for rapid detection of rotaviruses but virus isolation requires specific methods of cell culture and suitable cell lines. in this study, 41 samples were collected from diarrheal calves up to the age of one month, from indu...

during 2004-2006, a total number of 22 tissue samples obtained from poultry flocks suspected to respiratory diseases submitted to avian virology laboratory of faculty of veterinary medicine, were prepared for avian influenza virus (aiv) isolation in embryonated chicken eggs, according to the standard method. rt-pcr was established on tissue samples using specific primers for h9 gene. amplified ...

Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria. In addition to the phoP primers, the Hin and the H-1i primers, which tar...