Economizing the research protocols by using low cost technologies is the need of laboratories of developing world. Screening of recombinant E. coli colonies is the crucial step in gene cloning and expression studies. In the present study, the cost effectiveness of colony lysis method and colony PCR method in the screening of recombinant E. coli colonies was compared. The colony lysis method was...

A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition of the reaction by contaminants, and two consecutive PCRs, the second one with a nested primer, achieved the desired sensitivity. Comparison of the results obtained from stool swab samples processed...

We examine the formation of giant elliptical galaxies using their globular cluster (GC) systems as probes of their evolutionary history. The bimodal distributions of GC metallicities in such galaxies are often cited as evidence for the formation of giant elliptical galaxies through mergers involving gas-rich spirals, with the metal-rich GCs forming during the merger process. We explore an alter...

We study the stability of the giant gravitons in the string theory background with NSNS B field. We consider the perturbation of giant gravitons formed by a probe D(8− p) brane in the background generated by D(p− 2)D(p) branes for 2 ≤ p ≤ 5. We use the quadratic approximation to the brane action to find the equations of motion. The vibration modes for ρ , φ and r are coupled, while those of xk’...

Paying off the mortgage, once a widespread rite of passage for homeowners approaching retirement, has become less common in recent years. Concerns are mounting that the increased prevalence of housing debt among older homeowners could compromise financial security in retirement by expanding housing affordability problems, crimping essential nonhousing spending, increasing vulnerability to home ...