A solubilized preparation from pigeon liver microsomes produced four species of phosphatidate when incubated with a mixture of linoleoyland stearoyl-CoA and sn-glycero-3phosphate. The appearance of distearoyl (15%) and dilinoleoyl (42%) species indicates that either acid may acylate both positions 1 and 2. I-Stearoyl-Z-linoleoylglycero-3-phosphate (21%) was no more abundant than l-linoleoyl-2-s...

Lipid composition in cell membrane is closely associated with cell characteristics. Here, matrix-assisted laser desorption/ionization- Fourier transform ion cyclotron resonance mass spectrometry was employed to in situ determine membrane components of human mammary epithelial cells (MCF-10 A) and six different breast cancer cell lines (i.e., BT-20, MCF-7, SK-BR-3, MDA-MB-231, MDA-MB-157, and MD...