In mouse spleen cells, the effects of chloroplatinic acid and zirconium oxychloride on IgM antibody production against sheep red blood cells (SRBC) were studied in vitro. The following conclusion were drawn from this study: 1. Chloroplatinic acid suppressed direct plaque formation against SRBC in mouse spleen cells in vitro. 2. The viability of mouse spleen cells, to which chloroplatinic acid w...

The relative functional maturity of neonatal mouse spleen T- and B-cell populations was assessed by comparing the ability to respond to the thymic-independent antigen, DNP-Ficoll, or thymic-dependent SRBC by producing antibody in vitro. Although mouse spleen cells responded to DNP-Ficoll at an earlier age than they responded to SRBC or TNP-SRBC, the reason for the lag in the T-dependent respons...

The ability of IgM antibodies to specifically enhance the thymus-dependent humoral immune response to particulate antigens is well documented. We have used two approaches to test whether complement factors play a role in this process. First, mice were depleted of C3 by treatment with cobra venom factor (CVF) and then immunized with SRBC with or without IgM-anti-SRBC. CVF treatment severely impa...

Using mice double deficient for tumor necrosis factor (TNF) and lymphotoxin alpha (LT alpha), we demonstrated that TNF and/or LT alpha are necessary for development of a normal splenic microarchitecture and for isotype switch after immunization with sheep red blood cells (SRBC). In the present study, we extended these observations by determining which TNF receptor (TNFR) is involved in morpholo...

In studies designed to examine the effects of the immune system, recordings of multi-unit electrical activity (MUA) in the central nervous system were made in the preoptic area/anterior hypothalamus (POA/AH) and paraventricular nucleus (PVN) of rats, following sheep red blood cell (SRBC) immunization. Peak increases in POA/AH MUA were observed on the fifth day following SRBC sensitization, the ...

Variants of four hybridoma lines secreting antibodies specific for either trinitrophenyl (TNP) or sheep erythrocytes (SRBC) were isolated which have lost either the specific heavy (H) chain or the specific light (L) chain. They were fused to lipopolysaccharide-stimulated mouse spleen cells and the resulting secondary hybridomas were screened for the restoration of the original antibody specific...

BACKGROUND & OBJECTIVES The extracts from Caltha palustris L. have been shown to be beneficial for treating arthritis and rheumatism. In this study, the immunomodulatory effects of polysaccharide fractions B and C of C. palustris extracts were studied, using the collagen-induced arthritis (CIA) mouse arthritis experimental model. The aim was to determine the activity of blood phagocytic cells a...

The purpose of this study was to assess the effect of acupuncture on the immunological response. The induction of anti-sheep red blood cells (SRBC) plaque-forming cells (PFC) was used as a measurement of the immune response to treatment. In normal non-immunized mice, enhancement of PFC was seen after a single acupuncture treatment when spleen cells from stimulated mice were cultured with SRBC i...

This study reports the human red blood cells (HRBC) and sheep red blood cells (SRBC) membrane stabilizing property and the antioxidizing property of ethyl acetate soluble fraction of the gynaceum part of Bombax ceiba. The probable mode by which Bombax ceiba mediates its effects on inflammatory conditions was studied on HRBC & SRBC exposed to hypotonic solution. The results of the study revealed...

The feeding of sheep erythrocytes (SRBC) to mice leads to the production of two distinct T cell-derived suppressor factors by spleen cells. Each has been characterized for specificity, genetic restrictions, and cellular interactions. Fraction I has a 60,000-75,000 mol wt, is specific for antigen, and is suppressive of primary in vitro anti-SRBC responses at all times. It is not restricted by ma...