a new strategy for construction of synthetic gene encoding human basic fibroblast growth factor comprising dna annealing-ligation and augmentation by polymerase chain reaction was introduced. the sequence of the gene and corresponding amino acid chain were modified in order to increase stability of the protein. first, 300 bp and 160 bp fragments of the gene were assembled from 18 oligonucleotid...

objective: the incidence of breast cancer is approximately one million which makes this cancer one of the most common among women worldwide. breast cancer comprises 7% of the total death rate caused by cancers. several strategies that use tumor-associated antigen (taa) vaccination and early detection of breast cancer are clinically being developed. breast cancer is caused by increased over expr...

The G protein-coupled estrogen receptor GPER1/GPR30 is implicated in blood pressure regulation but the mechanisms are not identified. Here, we hypothesize that GPER1 controls blood pressure by regulating vascular smooth muscle cell Ca(2+) handling. Treatment with the GPER1 agonist G-1 (in the µM concentration range) acutely reduced spontaneous and synchronous Ca(2+) spike activity in A7r5 vascu...

background: radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (avm) may be used as markers for specific vascular targeting agents. in this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (pecam-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induc...

the pituitary-specific transcription factor (pit-1) gene is a candidate gene for growth, carcass and also for milk yield traits. in dairy farm animals, the main goal of the selection is the improvement of milk yield and composition. the genes of milk proteins and hormones are excellent candidate genes for linkage analysis with quantitative trait loci (qtl) because of their biological significan...

objective(s): streptokinase (ska) is an antigenic protein which is secreted by streptococcus pyogenes. streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. in the present study, recombinant streptokinase from s. pyogenes was produced and showed that recombinant ska protein was recognized by infected human sera using western blot analysi...

Abstract The need for high-affinity, SARS-CoV-2-specific monoclonal antibodies (mAbs) is critical in the face of global COVID-19 pandemic, as such reagents can have important diagnostic, research, and therapeutic applications. Of greatest interest ~ 300 amino acid receptor binding domain (RBD) within S1 subunit spike protein because its key interaction with human angiotensin converting enzyme 2...

Aim: Virus spike glycoprotein of SARS-CoV-2 is a good target for drug discovery. Objective: To examine the potential druggability protein pharmacophore-based discovery and to investigate binding affinity natural products with protein. Methods: Druggable cavities were searched though CavityPlus. A pharmacophore was built used hit identification. Autodock Vina evaluate hits' affinities. 10 chemic...

1. Plasticity at the connections between sensory neurons and their follower cells in Aplysia has been used extensively as a model system to examine mechanisms of simple forms of learning. Earlier studies have concluded that serotonin (5-HT) is a key modulatory transmitter and that it exerts its short-term actions via cAMP-dependent activation of protein kinase A. Subsequently, it has become cle...