GLIAL CELL LINE-DERIVED NEUROTROPHIC FACTOR REGULATION OF GENES ESSENTIAL FOR SELF-RENEWAL OF MOUSE SPERMATOGONIAL STEM CELLS IS DEPENDENT ON SRC FAMILY KINASE SIGNALING Jon M. Oatley*, Mary R. Avarbock, and Ralph L. Brinster From Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104 USA Running Title: GDNF regulated spermatogonial stem ...

background: the well documented source for adult multipotent stem cells is spermatogonial stem cells (sscs). they are the foundation of spermatogenesis in the testis throughout adult life by balancing self-renewal and differentiation. the aim of this study was to assess the effect of percoll density gradient and differential plating on enrichment of undifferentiated type a spermatogonia in diss...

Spermatogonial stem cell transplantation was first reported by Ralph Brinster's laboratory in 1994. It has proven to be a technological breakthrough in the study of both stem cells and Sertoli cell-germ cell interactions. This technique can be used to transfer testicular stem cells successfully from one animal to another of the same species (referred to as syngeneic transplants) and sometimes t...

Lin28a is a conserved RNA-binding protein that plays an important role in development, pluripotency, stemness maintenance, proliferation and self-renewal. Early studies showed that Lin28a serves as a marker of spermatogonial stem cells (SSCs) and promotes the proliferation capacity of mouse SSCs. However, there is little information about Lin28a in livestock SSCs. In this study, we cloned Capra...

Despite the presence of known mutagens and carcinogens in cigarette smoke, there is currently no evidence to show that smoking, or exposure to cigarette smoke, can result in heritable genetic mutation. We show that male mice exposed to mainstream tobacco smoke (MTS) exhibit a significant increase in germ-line mutation frequency in spermatogonial stem cells. We exposed mature male mice to MTS fo...

In recent years, embryonic stem (ES) cell-like cells have been obtained from cultured mouse spermatogonial stem cells (SSCs). These advances have shown that SSCs can transition from being the stem cell-producing cells of spermatogenesis to being multipotent cells that can differentiate into derivatives of all three germ layers. As such, they offer new possibilities for studying the mechanisms t...

Methods are detailed for isolating highly pure populations of spermatogonial stem cells from primary cultures of testis cells prepared from 22- to 24-day-old rats. The procedure is based on the principle that testicular somatic cells bind tightly to plastic and collagen matrices when cultured in serum-containing medium, whereas spermatogonia and spermatocytes do not bind to plastic or collagen ...