The present study was conducted to assess the fertilizing potential of frozen-thawed spermatozoa, which were cryopreserved after separation on a Percoll gradient, or washed out of seminal plasma. For this purpose, binding to the zona pellucida and other characteristics of the treated sperm cells were compared with those of cryopreserved spermatozoa from the same original sample which were not m...

The semen samples of 1,110 (one thousand, one hundred and ten) Igbo males attending infertility clinic, aged between 30-50 years were collected and analyzed. The specimens were collected and analysed in the microbiology laboratory of the University of Nigeria Teaching Hospital (UNTH) Enugu, Eastern Nigeria. The standard method of masturbation after 3-5 days of prior abstinence from sex before s...

We consider images of boar spermatozoa obtained with an optical phase-contrast microscope. Our goal is to automatically classify single sperm cells as acrosome-intact (class 1) or acrosome-reacted (class 2). Such classification is important for the estimation of the fertilization potential of a sperm sample for artificial insemination. We segment the sperm heads and compute a feature vector for...

This study was carried out to obtain informations regarding the effect of N-acetyl-D-glucosamine in the LEY (lactoseegg yolk) diluent according to incubation time in 5 ml maxi-straw and the effects of freezing rate, thawing temperature and thawing time in the LEN (lactose-egg yolk and N-acetyl-D-glucosamine) diluent on acrosome morphology and motility of frozen-thawed boar sperm. The study show...

The ultrastructure of normal human cilia and flagella was examined and quantitatively assessed to determine the normal variations in the structure of the axoneme. Ciliated respiratory epithelial cells and spermatozoa from 10 normal, nonsmoking male volunteers who had normal semen parameters were fixed for electron microscopy. Tannic acid and MgSO4 were included during fixation to enhance, in pa...

A total of 28 donor semen samples were used to evaluate the characteristics of laboratory variability in measuring reactive oxygen species (ROS). The objectives of this study were to assess the interassay (same sample observed on different days by the same observers) variability; interdonor, intraobserver (replications of the same sample on the same day) variability; and interobserver (multiple...

Background: There has been a growing claim that cell phone usage may have a detrimental effect on sperm chromatin. Infertile men possess anomalies in the composition of their sperm nuclei, displaying higher levels of loosely packaged chromatin and damaged DNA. In this study we examined the relationship of cell phone radiation and the protection effect of alpha_tocopherol on human sperm DNA. Mat...

The sperm chromatin structure assay (SCSA) was applied to human sperm, and factors affecting its routine operation investigated. The findings indicated that(a) non-sperm cells in semen can contribute to the results of the SCSA, (b) snap freezing in liquid nitrogen does not affect the result of the SCSA and is a good way of storing semen prior to testing, (c) there is good reproducibility when s...

OBJECTIVES To confirm or refute the hypothesis that organic farmers have higher sperm concentrations than traditional farmers. METHODS Traditional and organic farmers were selected randomly from central registers, and 171 traditional farmers and 85 organic farmers delivered one semen sample before the start of the spraying season. The participation rate was 28.8% among traditional farmers and...

The aim of the study was to determine the semen parameters of a proven fertile population and to compare these parameters with that of a subfertile group in the same region. Sixty-nine fertile male patients were studied and compared with 93 patients recruited at an infertility clinic. A sub-sample of patients was matched according to age. Sixty-one were studied in the fertile group and 62 in th...