background: recent studies have shown that benzene extract of ocimum sanctum (o. sanctum) leaves induces the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. objective: our aim was to investigate the effect of benzene extract of o.sanctum leaves on the cauda epididymal sperm paramet...

Spermatozoa are amongst the most variable cells, and three factors are thought to account for this variation in design: fertilization mode, phylogeny, and postcopulatory sexual selection. In addition, it has long been assumed that a tradeoff exists between sperm size and number, and although postcopulatory sexual selection affects both traits, empirical evidence for a tradeoff has so far been e...

BACKGROUND The aim of this study was to determine if only sperm with double stranded DNA could bind to the human zona pellucida (ZP). METHODS Sperm samples from 124 infertile men with a range of semen abnormalities were studied. Oocytes that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile s...

Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...

The research aims to evaluate proportion of X-Y chromosom bearing sperm after sexing local ram semen with different combination BSA concentration. object was ten ejaculated ram, three years old. design used CRD (completely randomize design) four treatments concentration on upper and bottom layer (T1: 3% & 6%, T2: 4% T3: 5% 10%, T4: 6% 12%), 10 repetitions. Data analysis using variance Dunca...

tau.amegroups.com © Translational Andrology and Urology. All rights reserved. The expert panel proposed a guideline on the clinical utility of sperm DNA fragmentation testing (1). Rapid advancement in the field of sperm DNA testing over the last two decades was summarized and structurally presented by the four clinical scenarios in an evidence-based approach. The role of male factor subfertilit...

Sperm competition is a prevalent phenomenon that drives the evolution of sperm function. High levels of sperm competition lead to increased metabolism to fuel higher sperm velocities. This enhanced metabolism can result in oxidative damage (including lipid peroxidation) and damage to the membrane. We hypothesized that in those species experiencing high levels of sperm competition there are chan...

Background: Danae racemosa is used medicinally and as a culinary spice. Its constituents are stated to have antithrombotic, antihepatotoxic, antinociceptive and antioxidant. Objective: Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. Methods: Wistar male rat (n=30) were allocated into thre...

BACKGROUND Protein tyrosine phosphorylation (TP) of human sperm is related to sperm capacitation and zona pellucida (ZP) binding. The aim of this study was to determine whether the TP of capacitated sperm is a useful marker for the ability of sperm to bind to the ZP and undergo the ZP-induced acrosome reaction (AR). METHODS Semen samples were obtained from 115 subfertile men with sperm count ...