Gamete recognition in the mouse is mediated, in part, by the binding of sperm surface galactosyltransferase (GalTase) to appropriate substrates in the egg zona pellucida. In this paper, sperm GalTase is shown to be an externally oriented, integral plasma membrane component. GalTase is not peripherally adsorbed to the cell surface, nor is it bound to cell surface glycoside substrates. GalTase ca...

Sperm nuclear migration during fertilization in Arabidopsis and rice has recently been found to be actin-dependent, but the driving force behind this actin cytoskeleton-dependent motion is unclear. Here, we confirmed that the actin-dependent sperm nuclei migration during fertilization is a conserved mechanism in plants. Using in vitro fertilization systems, we showed that a functional actin is ...

Background: It is showed that methamphetamine has harmful effects on male reproductive system. The aim of this study was to investigate the effects of methamphetamine administration on testis weight, sperm count, sperm morphology, and chromatin status as well as sperm apoptotis followed by the cytochemical tests in a mouse model. Materials and Methods: Twenty four NMRI male mice were divided in...

Human papillomaviruses (HPV) may cause sexually transmitted disease. High-risk types of HPV are involved in the development of cervical cell dysplasia, whereas low-risk types may cause genital condyloma. Despite the association between HPV and cancer, donor sperm need not be tested for HPV according to European regulations. Consequently, the potential health risk of HPV transmission by donor ba...

Background Sexing sperm is an important biotechnical that enables improve animal production, with achievement of maximum animal breeding. However, all sex semen methodologies can promote irreparable damages to sperm cell, which compromise their fertility [1]. Among the main causes of injures to the sperm are the reactive oxygen species, which have their production intensified during semen manip...

BACKGROUND Volume regulation is an important sperm function because defective sperm cannot negotiate the female tract in an infertile mouse model and swollen human sperm cannot penetrate and migrate through mucus. METHODS AND RESULTS The size of sperm from 52 donor ejaculates incubated in medium of female tract fluid osmolality (BWW290) was measured by flow cytometry to be identical to that i...

Abstract Background The selection of non-apoptotic sperm is related to successful fertilization. This study investigated the protective effects melatonin and role MACS (magnetically activated cell sorting) method prevent oxidative damage in vitro improve quality parameters such as viability DNA integrity. Materials methods Semen analysis was performed on 20 different eligible donors participati...

Background: Although AI practices have been introduced little over 60 years, the success rate remains relatively low. This might be due to the exclusive selection of semen based on motility analysis. Recent advancement in sperm sexing using flow cytometry with an increased throughput from next generation cell sorters, made use of this technology in studding sperm qualitative aspects other than ...

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets determine effectiveness adding sucrose tris-egg yolk on sperm swamp buffaloes produced by with egg albumin method. Semen was collected once week for 12 consecutive weeks artificial vagina from three male at Indonesian Center Animal Research Develop...