In this study, sperm characteristics (motility parameters and ionic composition of seminal plasma) and effect of ions on motility (duration of motility of sperm and percentage of motile spermatozoa) and fertilization capacity (fertilization rate, hatching rate, survival rate and larvae length) were investigated in Ctenopharyngdon idella. The longest duration of motility obtained in solution con...

In this study, sperm characteristics (motility parameters and ionic composition of seminal plasma) and effect of ions on motility (duration of motility of sperm and percentage of motile spermatozoa) and fertilization capacity (fertilization rate, hatching rate, survival rate and larvae length) were investigated in Ctenopharyngdon idella. The longest duration of motility obtained in solution con...

Apoptosis is common during spermatogenesis. Here, it was tested whether apoptosis could be induced in sperm after ejaculation. There were several lines of evidence to indicate that sperm are resistant to induction of apoptosis. First, incubation of bull sperm at temperatures characteristic of normothermia (38.5 degrees C) or heat shock (40 and 41 degrees C) for 4h did not increase the proportio...

When spermatozoa from two or more boars are mixed and females inseminated, the resulting litters are often skewed in favour of one male but there is currently no satisfactory physiological explanation for this effect. However, to reach the oocytes, the spermatozoa must enter the oviduct where they are exposed to factors that modulate their activity. They either become sequestered within the ovi...

BACKGROUND This study aimed to assess the relationship between apoptosis in human ejaculated spermatozoa, sperm morphology and the novel sperm deformity index (SDI). METHODS Semen specimens from 50 healthy donors were prepared by density-gradient centrifugation followed by incubating the prepared sperm with paramagnetic annexin V-conjugated microbeads and subjecting this to magnetic cell sort...

The integrity of the sperm genome and epigenome are critical for normal embryonic development. The advent of assisted reproductive technology has led to an increased understanding of the role of sperm in fertilization and embryogenesis. During fertilization, the sperm transmits not only nuclear DNA to the oocyte but also activation factor, centrosomes, and a host of messenger RNA and microRNAs....

Sperm collected from male fighting fish Betta splendens were activated in control water, water containing the ion-channel blocker gadolinium (a putative positive control), or water containing the isoflavone phytoestrogen genistein to determine the effects of acute genistein exposure on male reproductive function. Computer-assisted sperm analysis was used to quantify the proportion of sperm that...

background: artificial stimulation of mouse oocyte, in the absence of sperm contribution, can induce its parthenogenic activation of oocyte. ultrasound is one of the newest methods for artificial activation of mammal oocytes, and its successful utilization in pig oocyte activation has been recently reported. our objective was to assess the effect of ultrasound on mouse oocyte activation. materi...

Eukaryotic cilia and flagella are found on various types of cells, from single-cell protozoa to epithelial cells of multicellular organs. Most have a well-conserved structure called the axoneme, which generates ciliary and flagellar movements. The axoneme is a microtubule-based, highly organized structure composed of more than 250 kinds of proteins. Molecular motors, called the dyneins, exert f...