The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

Abstract Semen volume is the amount of ejaculate released by male Bali cattle during one ejaculation, which consists spermatozoa and seminal plasma. The purpose this study was to determine effect ejaculation on quality fresh frozen semen. collection carried out using an artificial vaginal method. collected semen then evaluated with microscopic parameters: motility, viability, abnormalities. cam...

Background: Osteopontin plays a crucial role in fertilization and subsequent embryonic development. Methods: The fresh semen samples were collected from crossbred Jersey Haryana breeding bulls. proteins the seminal plasma sperm membrane precipitated analysed by SDS-PAGE followed western blotting. Based on concentration of osteopontin plasma, bulls grouped as high (≥20 pg/ml), medium (10-20 pg/m...

A study was carried out to the effects of Ginger (Zingiber officinale) on quail semen quality, sexual hormones levels and histology testicles. total number 108 birds at age 8 weeks were divided into four groups, with three replicates per treatment 9 replicate. Birds in control group fed basal diets groups diet supplemented 0.25, 0.50 0.75% Ginger. At ages 10 till 20 weeks, blood samples collect...

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and hermaphrodites. In the earlier phase of gametogenesis, the germ cells of hermaphrodites differentiate into limited number of sperm--around 300--and are stored in a small 'bag' called the spermatheca. Later on, hermaphrodites continually produce oocytes....

Mapping epigenetic modifications and identifying their roles in the regulation of spermatogenesis and embryogenesis are essential for gaining fundamental medical understandings and for clinical applications. More and more evidence has shown that specific epigenetic modifications are established during spermatogenesis, which will be transferred into oocyte via fertilisation, and play an importan...

We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other ...

A 32 year old female presented with a history of 2 miscarriages at 6 weeks of pregnancy followed by 10 years of infertility. A bilateral wedge resection for polycystic ovaries had been carried out 8 years ago and had resulted in regular periods. A post-surgical laparoscopy revealed a blocked left tube and a tortuous right tube with multiple adhesions, which were partially freed. An unsuccessful...

The commonest problem related to the embryo itself is an abnormality of chromosomal number or aneuploidy. 95% of chromosomally abnormal embryos are abnormal as a result of a problem with the egg. The reason for this is that the egg does most of the work in accepting the DNA from sperm and then facilitating mixing of genes resulting in a new unique individual. Based on the age of the egg, the pr...

Unexplained male infertility is a condition in which infertile men have normal semen analyses on multiple occasions with no obvious physical or endocrine abnormality when female partner infertility has been ruled out. In addition to erectile problems and coital factors, immune causes and dysfunctional sperm may contribute to such condition. Contemporary andrology may help analyze the unexplaine...