Identification of microbial community members in complex environmental samples is time consuming and repetitive. Here, ribosomal sequences and hidden Markov models are used in a novel approach to rapidly assign fungi to their presumptive genera. The ITS1 and ITS2 fragments from a range of axenic, anaerobic gut fungal cultures, including several type strains, were isolated and the RNA secondary ...

the aim of this study was to determine the effect of yeast culture (saccharomyces cerevisiae sc 47) addition in the diet of dairy cows on their rumen fermentation and milk production. animals received a diet tmr based on good maize silage with a higher dry matter content (14 kg), 14 kg of lucerne-grass haylage, 5 kg of crushed ears of maize, 5 kg of beet pulp silage, 3 kg of crimped wheat, 2 kg...

this study was conducted to investigate the effect of biological treatment withpleurotus floridafungi on chemical composition and rumen dry matter (dm) and organic matter (om) degradability of wheat and barley straw. wheat and barley straw were collected from golestan province of iran and after pasteurization mixed with fungi spawn (than 5% by weight). after 21 days samples were dried in oven (...

PURPOSE The aim of this study was to determine the microbiology of primary acquired nasolacrimal duct obstruction (PANDO) and its antimicrobial susceptibilities. METHODS Ninety-three patients (100 eyes) diagnosed with PANDO, categorized as acute, chronic dacryocystitis, or simple epiphora, were prospectively enrolled. Lacrimal sac contents were cultured for aerobic and anaerobic bacteria and ...

Five strains of anaerobic fungi isolated from the faeces of wild (hog deer, Cervus porcinus; blackbuck, Antelope cervicapra; spotted deer, Axis axis; nilgai, Baselophus tragocamelus) and rumen liquor of domestic (sheep, Ovies aries) ruminants showing high fibrolytic enzyme producing ability were added to mixed rumen microflora of buffalo to study their effect on the digestibility of lignocellul...

There is an increasing need to identify alternative feeds for livestock that do not compete with foods for humans. Seaweed might provide such a resource, but there is limited information available on its value as an animal feed. Here we use a multi-omics approach to investigate the value of two brown seaweeds, Ascophyllum nodosum (ASC) and Laminaria digitata (LAM), as alternative feeds for rumi...

Two metabolism trials (experiments 1 and 2) were conducted to examine the effect of the organic S compound, sodium 3-mercapto-1-propane sulfonic acid (MPS) on feed intake, fiber digestibility, rumen fermentation and abundance of cellulolytic rumen microorganisms in cattle fed low S (<0.11%) roughages. Urea was provided in all treatments to compensate for the N deficiency (<0.6%) in the roughage...

We investigated the phylogenetic diversity and metabolic capabilities of members of the phylum Planctomycetes in the anaerobic, sulfide-saturated sediments of a mesophilic spring (Zodletone Spring) in southwestern Oklahoma. Culture-independent analyses of 16S rRNA gene sequences generated using Planctomycetes-biased primer pairs suggested that an extremely diverse community of Planctomycetes is...

anaerobic bacteria are well known causes of sepsis in adults but there are few studies regarding their role in neonatal sepsis. in an attempt to define the incidence of neonatal anaerobic infections a prospective study was performed during one year period. a total number of 400 neonates under sepsis study were entered this investigation. anaerobic as well as aerobic cultures were sent. the pati...