p. atlantica subsp. kurdica, with the local name of baneh, is a wild medicinal plant which grows in kurdistan, iran.  the identification of resistance gene analogs holds great promise for the development of resistant cultivars. a pcr approach with degenerate primers designed according to conserved nbs-lrr (nucleotide binding site-leucine rich repeat) regions of known disease-resistance (r) gene...

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...

More than 2,500 serovars of Salmonella species have been reported to date. A multiplex-PCR method was developed and evaluated for discriminating the four Salmonella enterica subsp enterica serovars, namely, S. Enteritidis, S. Typhimurium, S. Thompson and S. Infantis, most commonly isolated in Japan. Twenty-two serovars of 84 Salmonella strains and 7 species of non-Salmonella strains were evalua...

Kariyama and colleagues (5) recently described a multiplex PCR method for the detection of the vanA, vanB, vanC1, and vanC2/C3 genes, in addition to primers for the detection of the ddl genes of Enterococcus faecalis and E. faecium and of 16S rRNA (PCR control). However, the vanB primers used by these authors were derived from the sequence of the vanB1 subtype (1) and are incapable of detecting...

roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...