Protein S is a calcium-binding protein comprising two Greek key beta-barrel domains. We have used NMR and optical spectroscopies to show that, in the absence of calcium, the N-terminal domain of protein S forms two equilibrium folding intermediates that are in slow exchange. The intermediates arise from differential calcium-dependent folding of subdomains which are not contiguous along the poly...

abstract we had two cases of female complicated anorectal and genital malformations which underwent total mobilization of all structures of perinea to midline. case1: a 6 month old female with skin covered bladder, pubic diastasis, ectopic anus, vagina, and urethra to the right side of median cleft and lipoma on the left side. closure of pubic diastasis with iliac osteotomy and double barrel si...

Most algorithms in 3-D Computer Vision rely on the pinhole camera model because of its simplicity, whereas video optics, especially wide-angle lens, generates a lot of non-linear distortion. In some applications, for instance in stereo vision systems and robotic systems, this distortion can be critical. Camera calibration consists of finding the mapping between the 3-D space and the camera plan...

Transmembrane proteins play a major role in the normal functioning of the cell. Many transmembrane proteins act as a drug target and hence are of utmost importance to the pharmaceutical industry. In spite of the significance of transmembrane proteins, relatively few transmembrane 3D structures are available due to experimental bottlenecks. Due to this, it is imperative to develop novel computat...

urban spaces and in other words urban design and architecture have been found with close linkage with art and beauty especially the arts such as painting and sculpture. addressing beauty at different areas such as creation of galleries to offer works of art, addressing form of urban spaces and representing works of art in urban environment have improved social life and increased identity, conse...

BACKGROUND Based on our preclinical findings, we are assessing the efficacy of intratumoral injection of dendritic cells (DC) transduced with an adenoviral vector expressing the secondary lymphoid chemokine (CCL21) gene (Ad-CCL21-DC) in a phase I trial in advanced non-small cell lung cancer (NSCLC). While this approach shows immune enhancement, the preparation of autologous DC for CCL21 genetic...

Omp85 proteins mediate translocation of polypeptide substrates across and into cellular membranes. They share a common architecture comprising substrate-interacting POTRA domains, a C-terminal 16-stranded β-barrel pore and two signature motifs located on the inner barrel wall and at the tip of the extended L6 loop. The observation of two distinct conformations of the L6 loop in the available Om...

Clathrin triskelions can assemble into lattices of different shapes, sizes and symmetries. For many years, the structures of clathrin lattices have been studied by single particle cryo-electron microscopy, which probed the architecture of the D6 hexagonal barrel clathrin coat at the molecular level. By introducing additional image processing steps we have recently produced a density map for the...

Impact testing is a critical activity for many aerospace activities. Data on impacts can be employed to evaluate materials survivability, operations safety, and, if possible, plan prompt maintenance. A classical impact facility usually employs Light-Gas Guns (LGGs) the effect of collisions in controlled laboratory environment. In particular, single stage LGGs are relatively simple their working...