We have mapped the multiple (15-25) genes coding for the hormonally regulated major urinary proteins (MUPs) of the mouse by using a cloned cDNA probe. By Southern blot analysis of DNA from Chinese hamster-mouse somatic cell hybrids, all of the MUP genes were found to be on chromosome 4. Different inbred mouse strains showed DNA polymorphism in their MUP Southern hybridization pattern. Analysis ...

High-grade malignant nonHodgkin's lymphomas--five lymphoblastic, three pleomorphic, and two immunoblastic--developed in 10/25 cynomolgus monkeys (Macaca fascicularis) followed for up to 746 d after infection with simian immunodeficiency virus, strain SIVsm. These lymphomas were shown to be associated with an Epstein-Barr (EB)-like cynomolgus B-lymphotropic herpesvirus (CBLV) by electron microsc...

aspartic proteases are a relatively small group of enzymes which express in various nematodes including onchocerca volvulus. an estimation of the gene copy number corresponding to the ov7a clone, which contains a cdna insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of o. volvulus, was made by slot blot analysis in a closely related species o. gibsonig...

transgene integration and expression in host plant is quite unpredictable and is considered as the major problem in plant transformation. the variation in transgene copy number in transgenic plants influences the expression level and is one of such complication. in many plant species, the analysis of transgenic plants has shown that independent transgenic plants have one to many copies of trans...

An epidemiological survey of rheumatoid arthritis (RA) was undertaken in a tribal Xhosa community in the Transkei of Southern Africa. 577 respondents aged 18 and over were examined clinically and of these, 549 were investigated radiologically and 482 serologically. The presence of RA was then assessed by means of a modification of the Rome criteria, as used in previous comparable surveys. The p...

Bantu speech communities expanded over large parts of sub-Saharan Africa within the last 4000-5000 years, reaching different parts of southern Africa 1200-2000 years ago. The Bantu languages subdivide in several major branches, with languages belonging to the Eastern and Western Bantu branches spreading over large parts of Central, Eastern, and Southern Africa. There is still debate whether thi...

Characterization of genetically modified organisms through determination zygosity and transgene integration concerning both copy number genome site is important for breeding a transgenic line the use these in purpose which it was obtained. Southern blot, fluorescence situ hybridization or mating are demanding time-consuming techniques traditionally used characterization and, with exception mati...

The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridizatio...

Methods. Peripheral blood lymphocytes were prepared from 67 anonymous males. The cells were embedded in agarose and the genomic DNA digested with restriction enzyme Not I. The resulting DNA fragments were resolved on a contour-clamped homogeneous electric field gel, and the Not I fragment containing the red and green pigment genes was visualized by Southern blot hybridization with a human green...