Axotomy-induced increase in 2-deoxyglucose (2-DG) uptake by motor nuclei and neuronal chromatolytic changes were studied after subepineural injection of colchicine into the motor nerve. Hypoglossal nuclei of either cats or rats were axotomized bilaterally, while one of the nerves was injected with colchicine or saline proximal to the site of nerve transection and the other was left intact or in...

Cell membrane proteins synthesized after infection of Escherichia coli B with wild-type phage T4 and rIIA mutants were analyzed by dodecyl sulfatepolyacrylamide gel electrophoresis. A protein with an approximate molecular weight of 74,000 is present in membranes isolated from T4r+-infected cells, but is not found in membranes prepared from cells infected with an rIA mutant in which the major pa...

Rabbit lens epithelial cells display a similar "cobblestone" morphology and produce the same complement of sulphated macromolecules (also see Heathcote, J. G., and R. W. Orkin, 1984, J. Cell Biol., 99:852-860) whether grown on plastic or glass, dried films of gelatin or type IV collagen with laminin, or on gels of type I collagen. There was no evidence of basement membrane formation by these ce...

The objective of this study was to employ a tailor-made, surface-active agent (phosphonobile acid) in the design of dissolution media that more closely reflect various luminal fluid physicochemical parameters such as buffer capacity, osmolarity, surface tension, and pH. The proposed media are simple to prepare and use, and thus can be explored for routine application. Three BCS II drugs, glimep...

Models of the assembly of cytoskeletal and contractile proteins of eukaryotic cells require quantitative information about the rates of synthesis of individual component proteins. We applied the dual isotope technique of Clark and Zak (1981, J. Biol. Chem., 256:48634870) to measure the synthesis rates of cytoskeletal and contractile proteins in stationary and growing cultures of IMR-90 fibrobla...

White oils or waxes [mineral hydrocarbons (MHCs)] with substantial levels of saturated hydrocarbons in the range of C18 to C32 have produced hepatic microgranulomas and lymph node microgranulomas (also referred to as histiocytosis) after repeated administration to female Fischer-344 (F-344) rats. Female SpragueDawley (S-D) rats are less sensitive to these MHC-induced hepatic and lymph node effe...

Acid catalysed liquefaction of lignocellulosic materials in the presence of various alcohols is an efficient way to prepare alkoxy-glycosides and levulinic esters. In this mini review, the mechanism of the liquefaction process and the applications of liquefied products, such as upgrading into polyurethane and phenolic resol resin, are highlighted. INTRODUCTION The coming decades will be marked ...

Previous investigations have demonstrated that compounds capable of blocking presynaptic potassium channels can stimulate neurotransmitter release at both peripheral and central synapses. This study examined the in vitro effects of the “classical” potassium channel blockers 4-aminopyridine (4-AP) and tetraethylammonium (TEA) on the spontaneous basal release of [H]5-hydroxytryptamine ([H]5-HT) f...

The plasma 3H-radioactivity level showed a high value 1 hr after intra peritoneal injection of 3H-metaproterenol nd it l wered lineraly to reach 40% of the peak level 5 hr after administration. Radioactivity distributed to the kidneys and liver was markedly higher than that in other organs. Forty per cent of the amount given orally was excreted in the 24 hr urine specimen....

The effect of inhibitors of glutathione (GSH) synthesis, namely y-methyl glutamic acid, D-glutamic acid, cystamine, methionine-S-sulfoximine (MSX), buthionine-S-sulfoximine, and GSH itself, on the emission of H2S was investigated. All these compounds stimulated H2S emission from pumpkin (Cucurbitapepo L. cv Small Sugar Pumpkin) leaf discs in response to sulfate. MSX and GSH were the most effect...