The current study was designed to isolate and characterize urease-producing bacteria assess their ability precipitate calcium carbonate. Total eight were isolated from dung-rich soil samples collected Dakor, Gujarat. Out of these, two bacterial strains designated as DGDK-3 DGDK-4 found produce a considerable level urease in the initial screening on urea agar medium. Based morphological physiolo...

Helicobacter pylori, an important etiologic agent in a variety of gastroduodenal diseases, produces large amounts of urease as an essential colonization factor. We have demonstrated previously that urease is located within the cytoplasm and on the surface of H. pylori both in vivo and in stationary-phase culture. The purpose of the present study was to assess the relative contributions of cytop...

The influence of urease activity on N distribution and losses after foliar urea application was investigated using wild-type and transgenic potato (Solanum tuberosum cv Désirée) plants in which urease activity was down-regulated. A good correlation between urease activity and (15)N urea metabolism (NH(3) accumulation) was found. The general accumulation of ammonium in leaves treated with urea i...

Encouraging advances have been made in the application of microbial mineralization towards fixing and improving desertified sandy soils. However, desert soils arid areas exhibit high salinity that may limit urease activity production strains, thereby affecting solidification effects calcium binders saline In this study, a salt-tolerant strain (A80) produced was identified from Qaidam Basin. The...

To investigate the effects of different nutrient management regimes on the soil chemical, eco-enzymatic stoichiometric and microbial characteristics, soil samples were collected from a 30-year, long-term field experiment with six plots growing rice. The results showed that as integrated fertilization increased, so did the concentrations of soil total or available nutrients and microbial biomass...

An examination of in vivo polysome-bound activity indicates that soybean (Glycine max, cv. Prize) seed urease is synthesized on large polysomes (n >/= 15). In vitro urease synthesis is directed by a large RNA (3,000-3,300 nucleotides). Urease synthesis occurs throughout the normal protein biosynthetic phase of the developing seed. Surprisingly, the activity/antigen ratios of urease increase thr...

For studying the effect of soil fertility management practices on N mineralization, urease activity and maize yield, replicated field trials were established in 2015 at Misamfu Msekera agricultural research stations (ARS) representing two geo-climatic regions Zambia. The ARS is a sandy clay loam (SCL) from Paleustult, while that loamy sand (LS) Kandiustult. had three categories treatments namel...

Infection with Helicobacter pyloriis the most common cause of stomach and duodenal ulcers. About more than 80 % of people are infected with H. pylori in developing countries. H. pyloriuses urease enzyme product “ammonia” in order to neutralize and protect itself from the stomach acidic condition and urease enzyme activity has been shown to be essential to the colonization of H. pylori. Inhibito...

BACKGROUND Urease B is an important virulence factor that is required for Helicobacter pylori to colonise the gastric mucosa. Mouse monoclonal antibodies (mAbs) that inhibit urease B enzymatic activity will be useful as vaccines for the prevention and treatment of H. pylori infection. Here, we produced murine mAbs against urease B that neutralize the enzyme's activity. We mapped their epitopes ...