PURPOSE To investigate the effects of C-type natriuretic peptide (CNP) and sodium nitroprusside (SNP) on cyclic guanosine monophosphate (cGMP) accumulation and on carbachol (CCh)-stimulated inositol 1,4,5-triphosphate (IP3) production and contraction in ciliary muscle (CM) and iris sphincter (Sph) isolated from bovine and other mammalian species. METHODS Ciliary muscle and sphincter isolated ...

Using multiple interacting drugs to control both the mean arterial pressure (MAP) and cardiac output (CO) of patients with different sensitivity to drugs is a challenging task which this paper attempts to address. A multivariable model reference adaptive control (MRAC) algorithm is developed using a two-input, two-output patient model. The control objective is to maintain the homodynamic variab...

Objective of this study was to examine the effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor on steroid synthesis, growth and apoptosis of buffalo granulosa cells (GCs) in vitro. Follicular fluid of antral follicles (3-5 mm diameter) was aspirated and GCs were cultured in 0 (control), 10, 10, 10, 10 M of SNP for 48 h. To evaluate whether this effect was reversible, GCs were cultur...

We compared the effects of sodium nitroprusside (SNP), and of 8-bromo guanosine 3′,5′-cyclic monophosphate (8-BrcGMP), on the 4-aminopyridine (4-AP)-evoked Ca + -dependent release of glutamate from hippocampal nerve terminals and further investigated the role of protein kinase G (PKG) in this mechanism. SNP and 8-BrcGMP dose-dependently inhibited glutamate release, however SNP concentrations ([...

Metallothioneins (MTs) are small, cysteine-rich zinc binding proteins that are powerful antioxidants. In this study, we investigated the interaction between zinc, MTs, and other components of the antioxidant defense system in HepG2 cells. Cells were preincubated with zinc and then exposed to sodium nitroprusside (SNP), a nitric oxide (NO) donor. Both zinc pretreatment and SNP exposure separatel...

Our previous studies showed that nitric oxide (NO) could induce osteoblast apoptosis. MicroRNA-1 (miR-1), a skeletal- and cardiac muscle-specific small non-coding RNA, contributes to the regulation of multiple cell activities. In this study, we evaluated the roles of miR-1 in NO-induced insults to osteoblasts and the possible mechanisms. Exposure of mouse MC3T3-E1 cells to sodium nitroprusside ...