For the study of alkaline phosphatase systems a non-phosphate-containing buffer is required. The buffers in common use for the alkaline pH range are ammonia, borate, glytine and veronal. Ammonia and borate have a retarding effect on the rate of ,enzymic hydrolysis of phosphoric esters. Glycine activates phosphatase when present in very low concentrations (0 1-1 mm), but in the concentrations us...

The importance of neurofibrillar impregnations in the study of the nervous system needs no emphasis. However, the silver methods currently used involve such a complex of variables that the results are by no means constant. The present communication is devoted to the problem of interpretation of results, together with certain fundamental relations between brain tissue and silver solution. The mo...

We report the synthesis of a range sodium-carbonate co-substituted hydroxyapatite compositions with sodium and measured carbonate contents ranging from approximately 0.4–0.8 ​wt% 4.4–14.2 ​wt%, respectively, via aqueous precipitation reaction between calcium hydroxide, phosphoric acid either or hydrogen-carbonate. A subsequent heat treatment in dry CO2 at 600 ​°C allowed for Na–CO3 apatite cont...

After observations of cloudiness in the perfusion circuit at open intracardiac operations, laboratory experiments showed a precipitate in a Hartmann's solution (compound sodium lactate solution, Ringer-lactate) and sodium bicarbonate based priming fluid used for cardiopulmonary bypass. The precipitate was found to consist of calcium carbonate crystals. The crystals were not dissolved by adding ...