Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding field crops. Twenty-one microsatellite markers used to assess genetic diversity and relationship of 68 sunflower genotypes (Helianthus annuus L.). All of 21 pairs of SSR (Simple Sequence Repeats) markers produced a total number of 49 polymorphic bands. DNA fragments ranged from 9...

Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 a...

the genetic structure of the genus alburnus is not well known and the phylogenetic relationships among its species are uncertain. in the present study, simple sequence repeats (ssrs or microsatellites) were used to evaluate genetic diversity and genetic differentiation between alburnus mossulensis heckel, 1843 from kashgan river in lorestan province and alburnus caeruleus heckel, 1843 from gama...

four saudi arabian indigenous sheep populations including najdi, hbsi, arb, and naemi were genotyped for 16 microsatellite markers recommended by the food and agriculture organization (fao). this study aims to provide information on the genetic structure of the breeds analyzed and give indications and proposals for the cross breeding program. all loci were polymorphic in all populations and loc...

Sex determination in the Nile tilapia (Oreochromis niloticus) is thought to be an XX-XY (male heterogametic) system controlled by a major gene. We searched for DNA markers linked to this major locus using bulked segregant analysis. Ten microsatellite markers belonging to linkage group 8 were found to be linked to phenotypic sex. The putative Y-chromosome alleles correctly predict the sex of 95%...

objective(s): genetic analysis of two consanguineous pakistani families with localized autosomal recessive hypotrichosis was performed with the goal to establish genotype-phenotype correlation. materials and methods: genomic dna extraction had been done from peripheral blood samples. extracted dna was then subjected to pcr (polymerase chain reaction) for amplification. linkage analysis was perf...

In order to identify new markers around the glaucoma locus GLC1B as a tool to refine its critical region at 2p11.2-2q11.2, we searched the critical region sequence obtained from the UCSC database for tetranucleotide (GATA)n and (GTCT)n repeats of at least 10 units in length. Three out of four potential microsatellite loci were found to be polymorphic, heterozygosity ranging from 64.56% to 79.59...

The Chinese tree shrew (Tupaia belangeri yaoshanensis) has long been proposed to serve as an animal model for studying human diseases. However, its overall genetic diversity and population structure remain largely unknown. In the present study, we investigated the genetic diversity of population microsatellite DNA in wild Tupaia belangeri yaoshanensis. Sixteen microsatellite loci were assessed ...

PREMISE OF THE STUDY Fifty candidate microsatellite markers, generated using 454 shotgun sequencing, were tested for the widespread arctic/alpine herb Silene acaulis (Caryophyllaceae). METHODS AND RESULTS Fourteen out of 50 markers resulted in polymorphic products with profiles that enabled interpretation. The numbers of alleles per locus ranged from two to six, and the expected heterozygosit...

UNLABELLED PREMISE OF THE STUDY Vigna unguiculata is an economically important legume, and the complexity of its variability and evolution needs to be further understood. Based on publicly available databases, we developed chloroplast microsatellite primers to investigate genetic diversity within V. unguiculata and its related species Phaseolus vulgaris. • METHODS AND RESULTS Twelve polymo...