Introduction and Aim: Tuberculosis (TB) is a global health concern, claiming two million lives every year. Although an oldest known human infectious disease, researcher falling short of giving out effective reliable vaccine or therapy. The current antimycobacterial drugs include Isoniazid, Ethambutol, Rifampicin Pyrazinemamide available in market, but most these are to have certain adverse effe...

RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...