Huperzia serrata (Thunb. Ex Murray) Trevis belonging to the Lycopodiaceae family contains main active ingredient Huperzine A (HupA), which is effective in treatment of dementia. However, H. nature a low content HupA. Additionally, this plant grows very slowly and overexploited, thereby leading risk extinction. In study, we used vitro shoot induce multiplication by tissue culture method quantify...

A complete protocol is presented for in vitro regeneration of pomegranate (Punica granatum L.), a tropical fruit tree, using cotyledonary nodes derived from axenic seedlings. Shoot development was induced from cotyledonary nodes on Murashige and Skoog (1962) (MS) medium supplemented with 2.3±23.0 mM benzyladenine (BA) or kinetin (Kn). Both type and concentration of cytokinin signi®cantly in ̄uen...

A novel method of shoot regeneration from immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this explant, axenic cultures were established with very less contamination (10%). MS medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. Maximum number (35) of s...

Acacia auriculiformis, one of the short rotation and fast growing tree species is known for its pharmacological properties notably anti-helminthic, antifilarial, microbicidal, spermicidal activity etc. Conservation of Acacia auriculiformis is essential via ex situ plantation as the seed germination rate of this plant species has been reported very poor around 20-28%. Clonal multiplication forms...

In vitro propagation through nodal explants by indirect organogenesis of Clinacanthus siamensis Bremek. Nodal segments were surface sterilised in NaOCl for 5 min. This is followed by treating it with 0.1% Hgcl2 for 8 min. Different concentrations of BAP and 2P were added to full strength MS medium for culture initiation. Multiple shoots were formed in MS medium augmented with various concentrat...

An efficient and reproducible protocol for plantlet regeneration from nodal segments of Olive cv 'Frontio' has been developed. Media and explants browning due to exudation of phenolics from the explants were controlled by fortification of the medium with 100 mg/L ascorbic acid. Best establishment of olive explants was observed on half-strength MS salts fortified with 2.0 mg/L 6-benzylaminopurin...

An efficient micropropagation protocol of Cyclopia genistoides (L.) Vent., an indigenous South African shrub of economic importance, was established. In vitro shoot cultures were obtained from shoot tip fragments of sterile seedlings cultured on solid Schenk and Hildebrandt (SH) medium supplemented with 9.84 microM 6-(gamma,gamma-dimethylallylamino)purine (2iP) and 1.0 microM thidiazuron (TDZ)....

let r be a commutative ring with identity. let n and k be two submodules of a multiplication r-module m. thenn=im and k=jm for some ideals i and j of r. the product of n and k denoted by nk is defined by nk=ijm. inthis paper we characterize some particular cases of multiplication modules by using the product of submodules.

Centaurium erythraea plants obtained by indirect organogenesis are described in the paper. The plants were initiated from a single adventitious shoot regenerated from callus derived from the cotyledon of a 30-day-old seedling. The shoot was multiplied on MS medium supplemented with IAA (0.1 mg·L-1) and BAP (1.0 mg·L-1). The multiplication rate (28 shoots per culture within 4 weeks) was highest ...