A platform has been developed to measure the activity of 23 enzymes that are involved in central carbon and nitrogen metabolism in Arabidopsis thaliana. Activities are assayed in optimized stopped assays and the product then determined using a suite of enzyme cycling assays. The platform requires inexpensive equipment, is organized in a modular manner to optimize logistics, calculates results a...

zero tillage practices have a direct effect on soil microbial communities modifying soil productivity and sustainability. the crop sequences used can change the aforementioned properties, too. in this study, we evaluated the effect of crop sequences under zero tillage management on soil biological and chemical properties including vertical distribution of soil organic carbon, soil basal respira...

background: long-term glucocorticoid therapy causes secondary osteoporosis leading to pathological fractures. glucocorticoid action in bone is dependant upon the activity of 11β-hydroxysteroid dehydrogenase type 1 enzyme (11β-hsd1). piper sarmentosum is a local herb that possesses the ability to inhibit 11-βhsd1 enzyme activity. we aimed to determine the effects of piper sarmentosum water extra...

A method for enzymatic preparation of 3-dehydroquinate and 3-dehydroshikimate in the shikimate pathway was established by controlling the enzyme activity of 3-dehydroquinate dehydratase. When quinate was incubated with the membrane fraction of acetic acid bacteria at pH 5.0, 3-dehydroquinate was formed as the predominant product. 3-Dehydroshikimate was the sole product when incubated at pH 8.0....

In the rat, shikimate was metabolized and excreted as hippurate, hexahydrohippurate, 3,4,5,6-tetrahydrohippurate, t-3,t-4-dihydroxycyclohexane-r-1-carboxylate and c-3,t-4-dihydroxycyclophexane-r-1-carboxylate, conjugates of catechol and CO2. The metabolism was entirely dependent on various initial microbial transformations in the gut, metabolite formation being suppressed in animals pretreated ...

The effect of various NaCl treatments (0, 50, 100, 200 and 300 mM) at different growth and development stages (tillering, boot swelling, flowering and polination) of two wheat cultivars (Ghods : salt-sensitive; Boolani : salt-resistant) on proline concentration and the kinetic activity of proline dehydrogenase was studied under greenhouse conditions. Generally, in response to salinity treatment...

Introduction: Recently depleted uranium is being widely used as anti-armour ammunition and at very high temperature, results in information of an aerosol of very small uranium oxide particles, which may be inhaled. It is alleged that these particles represent a new battlefield hazard because of the chemical toxicology and/or radioactivity. Method & materials: Male Wistar strain albino rats ...

Due to the emergence of resistance toward current antibiotics, there is a pressing need to develop the next generation of antibiotics as therapeutics against infectious and opportunistic diseases of microbial origins. The shikimate pathway is exclusive to microbes, plants and fungi, and hence is an attractive and logical target for development of antimicrobial therapeutics. The Gram-positive co...