We describe here an approach for the isolation and characterization of substrate attached membranes of cultured cells. The procedure for ventral membrane preparation is based on a short incubation with ZnCl,, followed by shearing with a stream of buffer. By varying the intensity of shearing it was possible to obtain reproducibly either entire ventral membranes or highly enriched focal contacts....

Abderitid marsupials are common in vertebrate-bearing deposits from the middle Miocene of Argentine Patagonia. Recent collections from the inland Pinturas Formation and slightly younger coastal Santa Cruz Formation have dramatically increased the number of abderitid specimens. These new collections permit a re-assessment of abderitid taxonomy as well as an investigation of the dietary habits of...

Measurements of the yield behaviour of a cohesive bulk solid in the Jenike shear tester are normally done in two steps. First, pre-shearing to a steady state yielding, and secondly, shearing under a lower normal stress to establish the shear stress at incipient failure. Due to the limited strain of the sample in this apparatus, the material must be consolidated in an earlier stage. This is done...

The mechanical response of clay mass can be significantly affected by the distribution and arrangement of the particles and the interaction between them. A series of compression and extension tests under drained and undrained conditions were performed on normally consolidated and heavily over-consolidated clay specimens with different microfabric. The experimental data indicates strong influenc...

* Author for correspondence: Dr. Maha Abdullah, Immunology Unit, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Tel.: 603-89472375, Fax: 603-89412787, Email: [email protected]. Abstract. Next-generation sequencing (NGS) technologies, capable of sequencing genomic DNA and RNA at high throughput with unprecedented speed, hav...

A computer-controlled display of random dots was used to study perceptions of depth. In this display, a field of stationary random dots surrounded a rectangular area in which random dots moved with uniform velocity in a single direction. The boundaries of this rectangle did not move. When dot motion was perpendicular to the longer boundary of the rectangle (occluded motion), the rectangle seeme...