An enzyme-linked immunosorbent assay (ELISA) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. The assay showed improved sensitivity over the virus neutralization (VN) test and over an enhanced VN test in which incubation of antibody-virus mixtures was carried o...

A dengue plaque reduction neutralization test (PRNT) to measure dengue serotype-specific neutralizing antibodies for all four virus serotypes was developed, optimized, and validated in accordance with guidelines for validation of bioanalytical test methods using human serum samples from dengue-infected persons and persons receiving a dengue vaccine candidate. Production and characterization of ...

BACKGROUND: Infectious Bovine Rhinotrachitis (IBR) caus-ed by Bovine Herpes Virus-1 (BoHV-1) was first, observed in one of the Tehran Industrial cattle breeders, with importation of cattle in 1965. Later, the isolation of bovine herpes virus approved the existence of the disease in the most of the provinces of the Iran. OBJECTIVES: Presence of antibodies against BoHV-1 was evaluated in young bo...

The antigenic relatedness of minute virus of mice (MVM), Kilham rat virus (KR), H1 virus (H1), haemorrhagic encephalopathy of rats virus (HER), porcine parvovirus (PPV), canine parvovirus (CPV), feline panleukopenia virus (FPV), goose parvovirus (GPV) and bovine parvovirus (BPV) was studied by immunofluorescence microscopy (FA) and by serum neutralization (SN). An antigenically related group co...

A comparison has been made of the neutralizing value of pooled convalescent monkey serum for the filtered virus of poliomyelitis, before and after a series of reinforcement injections of the same virus strain. The strength of the pooled convalescent serum is increased by the reinforcing procedure. The original monkey convalescent serum had a neutralization value much below that of a pooled huma...

Goose astrovirus (GoAstV) has frequently been isolated in China since it was first identified as the etiological agent of visceral gout goslings 2017. However, actual prevalence GoAstV infection and its economic impact on commercial goose production remain poorly characterized. Here, virus detection serological testing were conducted to determine extent flocks. We detected RNA 2% (6/300) dead-i...