Two age groups (young and old, n = 12) matched for physical fitness and two physical fitness groups (high and old, n = 12) matched for age participated in a four month physical fitness programme. Blood samples were drawn at four stages of metabolic stress at the pre-test and five stages at the post-test. The blood samples were analyzed by calorimetric methods for total cholesterol and free chol...

Use of serum-free media and the related genera of animal-free and protein-free media has increased significantly over the past 15 years. This is particularly true in industrial applications, in which the use of serum presents a safety hazard and a source of unwanted contamination in the production of biopharmaceuticals (1). As the name suggests, serum-free media are prepared without animal seru...

References 1. Bry L, Chen PC, Sacks DB. Effects of hemoglobin variants and chemically modified derivatives on assays for glycohemoglobin [Review]. Clin Chem 2001;47:153–63. 2. Sacks DB. Hemoglobin variants and hemoglobin A1c analysis: problem solved? [Editorial]. Clin Chem 2003;49:1245–7. 3. Watanabe T, Kato K, Yamada D, Midorikawa S, Sato W, Shiga M, et al. A nondiabetic case of hemoglobin var...

Cadmium (Cd) is an industrial pollutant and carcinogenic metal. Most in vitro Cd toxicity studies have been carried out in various cell lines cultured in 10% fetal bovine serum (FBS) containing medium. In this report, we compared the toxic effect of Cd (0-300 microM) on cell growth, total RNA, total proteins, and antioxidant enzymes in rat normal liver cells cultured in medium with 10% FBS or c...

When concentrations of free thyroid hormones in serum are measured by equilibrium dialysis, the serum sample is diluted by dialysis buffer, resulting in a new equilibrium for distribution of these hormones that differs from that in the original serum sample. We have derived a mathematical formulation, based on an equilibrium model for multiple ligands and multiple binding sites, to determine th...

The ability to culture hematopoietic cells in readily characterizable and scalable stirred systems, combined with the capability to utilize serum-free medium, will aid the development of clinically attractive bioreactor systems for transplantation therapies. We thus examined the proliferation and differentiation characteristics of peripheral blood (PB) mononuclear cells (MNC), cord blood (CB) M...