To study the complete natural process of a Mycoplasma pneumoniae outbreak in a semiclosed room such as a primary school room, we investigated two separate M. pneumoniae outbreaks involving 81 students in total in two primary schools in Hangzhou, China. M. pneumoniae isolates from pharyngeal swabs were detected by fluorescence quantitative real-time PCR (RT-PCR) and culture. The class in school ...

We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV GGI RT-PCR was determined by probit analysis with...

Little information on the evaluation of airborne particulate matter (APM) and sedimentation particles from subway stations is available. The thermal metamorphism of train wheels generating toxic particles in subway stations is a possibility. In this study, the toxicity and physiological effects of particles from subway stations were evaluated using a yeast bioassay system. Estrogenic and anties...

This study was designed to assess operating room contamination with nitrous oxide and halothane when nitrous oxide 3 1/min and oxygen 2 1/min containing halothane one per cent were passed into a semiclosed circle absorber system from which the patients' lungs were ventilated with an Ohio ventimeter through a cuffed tracheal tube, with the exhaled gas vented to the floor. The normal room ventila...

This study evaluated the distribution of polychlorinated biphenyls (PCBs) in 39 surface sediment samples and four cores collected in Toulon Bay, a semiclosed area submitted to various anthropogenic inputs. The concentration of PCBs in the superficial sediment samples ranged from 1.7 to 2530 ng g(-1) dry weight. The spatial distribution of these compounds suggested that the high concentrations o...

based on the point of view of geometrical representation of an intuitionistic fuzzy set, we take into account all three parameters describing intuitionistic fuzzy set, propose a kind of new method to calculate correlation and correlation coefficient of intuitionistic fuzzy sets which is similar to the cosine of the intersectional angle in finite sets and probability space, respectively. further...