A semisolid brucella medium with 10% sheep blood was used for storage and transport of Campylobacter fetus subsp. jejuni and subsp. intestinalis and kept isolated alive about 3 weeks or longer at 25 degrees C or when sent through the regular mail.

Many methods have been proposed for the isolation of temporarily suppressed phases of diphasic Salmonella (Craigie, 1931; Gard, 1938; Hajna, 1944). These absorption methods are effective but require incubation for at least two days and considerable manual labor. Juenker (1946) suggested a procedure by which broth was inoculated with the organisms of the desired phase by passing them through a s...

[Purpose] This study investigated the effect of a combined method incorporating the Mendelsohn maneuver and effortful swallowing on aspiration in patients with dysphagia after stroke. [Subjects and Methods] Three patients with dysphagia were recruited. All patients were treated with a combined method consisting of the Mendelsohn maneuver and effortful swallowing. The intervention period was tot...

Preliminary experiments revealed that coagulase positive staphylococci could be distinguished culturallv from coagulase negative staphylococci in a soft agar medium containing a small amount of normal human or rabbit plasma. In a medium of suitably low viscosity, staphylococci produce elongated diffuse feathery colonies. When coagulable plasma is incorporated in such a medium, the colonies of c...

Foreign body aspiration occurs when a solid or semisolid object becomes lodged in the larynx or trachea. It can be a life-threatening emergency, especially if it is large enough to occlude the airway. However, small aspirated objects may go unnoticed until symptoms occur. Therefore, it is frequently misdiagnosed. A high level of clinical suspicion, patient's risk factors, and thorough history a...

Objectives: A multi-site study evaluated the clinical performance of the Roche LightCycler PCR assay in the detection of methicillin-resistant Staphylococcus aureus (MRSA) in comparison to routine chromogenic agar culture method (BD BBL CHROMagar II). Two other variables were also investigated: to establish if screening the throat increases the detection rate of MRSA colonisation; and, to deter...

OBJECTIVE The study aimed to examine secular trends and determinants of changes in complementary feeding indicators in Nigeria. DESIGN, SETTING AND PARTICIPANTS Data on 79 953 children aged 6-23 months were obtained from the Nigeria Demographic and Health Surveys (NDHS) for the period spanning 2003-2013. The surveys used a stratified two-stage cluster sample of eligible mothers aged 15-49 yea...

When small tubes (75 by 10 mm), containing 1 or 2 ml of SIM medium (Difco) or Trypticase Agar Base (semisolid) (BBL), are preheated to 37 C, "stabinoculated" from a young agar slant culture, and incubated in a water bath at 37 C, motility may be observed in a fraction of the time required by the methods usually employed with these media. In a series of cultures which were studied, motility coul...

The objective of this study was to produce an economical, easy to prepare, field-suitable enrichment medium for detection of Campylobacter jejuni in small numbers. A semisolid aerobic enrichment medium was developed. Rates of recovery from inoculated medium, sterile swabs, and mixed cultures of C. jejuni and coliform bacteria were tested.

The aim of this study was to evaluate the release of rosmarinic acid (RA) from the experimental topical formulations with the Melissa officinalis L. extract and to evaluate its penetration through undamaged human skin ex vivo. The results of the in vitro release study showed that higher amounts of RA were released from the emulsion vehicle when lemon balm extract was added in its dry form. An i...