BACKGROUND & OBJECTIVES As there are no standard laboratory techniques for the rapid detection of Pneumocystis jirovecii in India, this study was undertaken to evaluate and establish an optimal and rapid technique for the detection of P. jirovecii by comparing three different techniques - staining technique, application of a real time polymerase chain reaction (RT-PCR) targeting kex 1 gene and ...

background and objectives: hepatitis c virus (hcv) is a major public health problem worldwide. replication and persistence of hcv genome have been described in the liver tissue as well as b cells lymphocyte. several investigations have reported that long-term persistence of hcv in b cells may result in hodgkin and non-hodgkin lymphoma. this study was aimed to determine frequency of hcv rna in h...

BACKGROUND AND PURPOSE Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. MATERIALS AND METHODS Sampling was conducted in...

Theileria annulata, as a pathogenic agent of tropical theileriosis, gives rise annually to serious economic losses in cattle industry of Iran. The carrier cattle, harbouring the latent forms of Theileria annulata, play a major role in infecting tick vectors and in disseminating the infection. The aim of this study was determining the carrier cattle, infected with Theileria annulata in Golestan ...

A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen....

Identification of Cutaneous Leishmaniasis-causing Species Using Semi-Nested PCR in Kohgiluyeh and Boyer-Ahmad Province During 2013-2019

The incidence and prognostic relevance of bone marrow (BM) and leukapheresis (PBPC) tumor cell contamination (TCC) in breast cancer patients is still to be circumstantiated. We developed a new comprehensive gene expression panel to study cytokeratins (CK), maspin (MAS) and mammaglobin (MAM) as possible predictors of prognosis. Forty-eight patients undergoing high dose chemotherapy (HDCT) and PB...

Amplification of human immunoglobulin has many potential applications such as analysis of clonality, isolation of immunogenic antigens and antigen-specific immunotherapy. Here we describe a method for amplification of human immunoglobulin heavy and light chains from single B lymphocytes or plasma cells. Cells are isolated by FACS, and Ig is amplified by semi-nested RT-PCR. The method is versati...