leptospirosis is a zoonosis of worldwide distribution, caused by leptospira interrogans and is considered as an emerging global public health problem. transmission usually results from direct or indirect exposure to the urine or other body fluids of leptospiruric animals which may become a source of infection for human or other animals. having a humid climate with plenty of annual rainfall, gui...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...

Mycoplasma gallisepticum is the most important and infectious Mycoplasmosis. It is caused lots of economic losses for poultry's industry of Iran. The target of this study is comparison of culture and nested PCR techniques to detect Mycoplasma gallisepticum infection of chicken’s from industrial farms in Kerman province of Iran. 88 isolates received from industrial far...

BACKGROUND Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences. This approach, however, is difficult when the amount of DNA is too low to be amplified by standard PCR. Nested PCR can be employed as it can amplify samples with DNA concentration several-fold lower than standard PC...

Mycoplasma gallisepticum is the most important and infectious Mycoplasmosis. It is caused lots of economic losses for poultry's industry of Iran. The target of this study is comparison of culture and nested PCR techniques to detect Mycoplasma gallisepticum infection of chicken’s from industrial farms in Kerman province of Iran. 88 isolates received from industrial far...

The objective of this study is to investigate and compare the sensitivity in conventional PCR, quantitative real time PCR, nested PCR and western blots for detection of prostate cancer tumor markers using prostate cancer (PCa) cells. We performed conventional PCR, quantitative real time PCR, nested PCR, and western blots using 5 kinds of PCa cells. Prostate specific antigen (PSA), prostate spec...

Background: Spermatogenesis associated 19 (Spata19) was introduced as a testis-specific gene that probably involved in spermatogenesis cell apoptosis. Therefore, this study aimed to investigate the effect of Spata19 inactivation on sperm count. Materials and Methods: We generated global knockout mice by CRISPR/Cas9 nickase technology. Disability validated three levels DNA, RNA, protein using PC...