The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

Antibiotics are added to semen extenders to take care of heavy microbial load, however, their continuous use poses a constant threat of developing antibiotic resistance by the common microbes present in the semen. Our hypothesis was that natural honey, having antibacterial activity and rich in fructose could replace the use of antibiotics and fructose in the semen extender. Twenty-four ejaculat...

A study was conducted to investigate the various glycerol concentrations on freezability of mithun semen. The present study was conducted in semen production laboratory, ICAR-NRC on Mithun, Medziphema, Nagaland, India. A total of 20 ejaculates were collected from apparently healthy mithun bulls (n=6) by trans-rectal massage method. These ejaculates were extended with tris–egg yolk-citrate-glyce...

The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...

Background: The importance of polyunsaturated fatty acids (PUFAs) on chilled sperm quality is under investigation. Addition of fatty acids to the diet can influence sperm quality and male fertilizing capacity. The aim of this study was to investigate if the addition of n-3 PUFAs improves quality of bovine chilled sperm. Materials and Methods: In order to do experiment, 5 proven bulls were rando...

OBJECTIVE The potential of NaHCO3 versus human serum to induce germ tube formation in Candida albicans was investigated. SPECIMENS A total of 100 isolates were obtained from oral swabs of patients presenting with thrush. Approval for the study was granted by the Joint Research Ethics Committee (JREC/23/08). METHOD Confirmed C. albicans isolates by routine methods were tested for germ tube i...

Low-Density Lipoprotein (LDL) extracted from egg yolk has recently known can eliminate the adverse effect associated in use of fresh yolk. The role LDL liquid preservation at 4°C ram sperm not been explored. This research evaluates effects substituting with 4 °C on 5 days. objective this was to assess for as an extender 4°C, well spermatozoa motility, viability, morphology, plasma membrane, and...

Computer-assisted sperm analyzers (CASA) have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Mammalian spermatozoa experience osmotic stress when the glycerol is added to the cells prior to freezing and removal from the cells after thawing. In order to minimize osmotic damage, cryoprotectants having lower mol...

experiment was conducted to study the effect of different antibiotics on sperm quality of ram by a completely randomized block design using 23 factorial arrangement with 2 block and 4 observations in each experimental units. factors include three antibiotics named gentamycin (g), lincomycin (l) and ampicillin (a) that were used at two different levels including zero and, respectively 5, 10 and ...

AIM To evaluate two extenders and two cryoprotectant agents (CPA) for alpaca semen cryopreservation. METHODS Semen samples were obtained from four adult alpacas (Lama pacos) and frozen using extender I (TRIS, citrate, egg yolk and glucose) or extender II (skim milk, egg yolk and fructose), each containing either glycerol (G) or ethylene glycol (EG) as CPA. Consequently, four groups were forme...