A continuous decline in the number and range of capercaillie (Tetrao urogallus L.) in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryo...

Mycobacterium avium subspecies paratuberculosis was identified by nested polymerase chain reaction in the blood and semen of a 4year-old Holstein breeding bull that exhibited clinical signs of paratuberculosis and was serologically positive for the organism by agar gel immunodiffusion and ELISA, although three fecal cultures were negative for the organism. The animal subsequently died, but a ne...

The present investigation aimed to conduct a genetic study of dairy cattle and buffalo bulls based on economic traits and their phenotypic correlation. The means of various economic traits in both the species are presented. Cattle bulls reported a better libido score, Flehmen response, requirement of mounting stimulus and semen volume, conception rate and expected predicted difference for milk ...

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

Semen collection evaluation and addition of preservatives to increase storage period of sperm are essential for successful artificial insemination. This study was conducted on 4rams (2 Ghezel Merinos and 2 Merinos Moghani) to evaluate the effect of two diluents in Khalatposhan research station. Average age of rams was 3 years. Rams were trained to serve the artificial insemination and semen sam...

Aims: To find out the percentage of live spermatozoa after diluting goat's liquid semen using an andromed extender plus sweet orange essential oil.
 Study Design: Randomized Block Design.
 Place and Duration Study: Sample: Galang, Deli Serdang Regency, Indonesia, between December 2022 January 2023.
 Methodology: The research procedure starts with preparation extender, collection ...

The types and rates of acrosome and other (head, mid-piece and tail) abnormal spermatozoon types were determined by the Spermac staining technique. Semen from 5 stray tom cats under the same management conditions was used. Semen collection was performed under general anesthesia by electro-ejaculator once a week for 5 weeks. After ejaculation the semen was diluted by 100 μl of 0.9% NaCl solution...

The WHO “Laboratory manual for the Examination and processing of human semen” is a reference guide, firstly published in 1980, to standardize the procedures for the examination of human semen. It has been updated three times; the last version was published in 2010 (1) It provided guidance about many aspect of semen examination like: semen collection, macroscopic and microscopic examinations, im...

ing (eg, a rug) rather than on a slippery surface or table. Care should be taken to intimidate the dog as little as possible; lifting the dog to an examination table, giving injections, etc, should be performed after the semen is collected. Semen may be collected in the absence of a bitch (although sperm numbers may be lower), but the presence of a bitch is preferable. The pheromone methyl para...

The aim of the experiment was to determine the effect of potential antioxidants (adenosine, L-cysteine hydrochloride, ascorbic acid, magnesium fumarate and prolactin) supplementing the Biosolwens extender on semen survival time and sperm chromatin structure. The semen motility was examined every day and the susceptibility of sperm chromatin to denaturation was evaluated on collection day and da...